Počet záznamů: 1
Subcellular spatial resolution achieved for deep-brain imaging in vivo using a minimally invasive multimode fiber
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SYSNO ASEP 0499922 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Subcellular spatial resolution achieved for deep-brain imaging in vivo using a minimally invasive multimode fiber Tvůrce(i) Vasquez-Lopez, S.A. (GB)
Turcotte, R. (GB)
Koren, V. (GB)
Ploschner, M. (GB)
Padamsey, Z. (GB)
Booth, M. (GB)
Čižmár, Tomáš (UPT-D) RID, ORCID, SAI
Emptage, N.J. (GB)Celkový počet autorů 8 Číslo článku 110 Zdroj.dok. Light-Science & Applications. - : Springer - ISSN 2047-7538
Roč. 7, DEC (2018)Poč.str. 6 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova light ; microscopy ; system ; limits ; mice Vědní obor RIV BH - Optika, masery a lasery Obor OECD Optics (including laser optics and quantum optics) CEP EF15_003/0000476 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UPT-D - RVO:68081731 UT WOS 000453577600002 EID SCOPUS 85058859483 DOI 10.1038/s41377-018-0111-0 Anotace Achieving intravital optical imaging with diffraction-limited spatial resolution of deep-brain structures represents an important step toward the goal of understanding the mammalian central nervous system(1-4). Advances in wavefront-shaping methods and computational power have recently allowed for a novel approach to high-resolution imaging, utilizing deterministic light propagation through optically complex media and, of particular importance for this work, multimode optical fibers (MMFs)(5-7). We report a compact and highly optimized approach for minimally invasive in vivo brain imaging applications. The volume of tissue lesion was reduced by more than 100-fold, while preserving diffraction-limited imaging performance utilizing wavefront control of light propagation through a single 50-mu m-core MMF. Here, we demonstrated high-resolution fluorescence imaging of subcellular neuronal structures, dendrites and synaptic specializations, in deep-brain regions of living mice, as well as monitored stimulus-driven functional Ca2+ responses. These results represent a major breakthrough in the compromise between high-resolution imaging and tissue damage, heralding new possibilities for deep-brain imaging in vivo. Pracoviště Ústav přístrojové techniky Kontakt Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Rok sběru 2019
Počet záznamů: 1