Počet záznamů: 1
Chromatin architecture changes and DNA replication fork collapse are critical features in cryopreserved cells that are differentially controlled by cryoprotectants
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SYSNO ASEP 0494984 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Chromatin architecture changes and DNA replication fork collapse are critical features in cryopreserved cells that are differentially controlled by cryoprotectants Tvůrce(i) Falk, Martin (BFU-R) RID, ORCID
Falková, Iva (BFU-R) ORCID
Kopečná, Olga (BFU-R) ORCID
Bačíková, Alena (BFU-R)
Pagáčová, Eva (BFU-R) ORCID
Šimek, Daniel (FZU-D) RID, ORCID
Golan, Martin (FZU-D) RID
Kozubek, Stanislav (BFU-R) RID
Pekarová, Michaela (BFU-R) RID
Follett, S.E. (US)
Klejdus, B. (CZ)
Elliott, K.W. (US)
Varga, K. (US)
Teplá, O. (CZ)
Kratochvílová, Irena (FZU-D) RID, ORCID, SAICelkový počet autorů 15 Číslo článku 14694 Zdroj.dok. Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 8, OCT 2 2018 (2018)Poč.str. 18 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova human spermatozoa ; oxidative-stress ; damage response ; repair ; trehalose ; integrity ; dynamics ; sperm ; fragmentation ; instability Vědní obor RIV BO - Biofyzika Obor OECD Cell biology Vědní obor RIV – spolupráce Fyzikální ústav - Biofyzika CEP NV16-29835A GA MZd - Ministerstvo zdravotnictví GA16-12454S GA ČR - Grantová agentura ČR LO1409 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora BFU-R - RVO:68081707 ; FZU-D - RVO:68378271 UT WOS 000446035900024 EID SCOPUS 85054195372 DOI 10.1038/s41598-018-32939-5 Anotace In this work, we shed new light on the highly debated issue of chromatin fragmentation in cryopreserved cells. Moreover, for the first time, we describe replicating cell-specific DNA damage and higher-order chromatin alterations after freezing and thawing. We identified DNA structural changes associated with the freeze-thaw process and correlated them with the viability of frozen and thawed cells. We simultaneously evaluated DNA defects and the higher-order chromatin structure of frozen and thawed cells with and without cryoprotectant treatment. We found that in replicating (S phase) cells, DNA was preferentially damaged by replication fork collapse, potentially leading to DNA double strand breaks (DSBs), which represent an important source of both genome instability and defects in epigenome maintenance. This induction of DNA defects by the freeze-thaw process was not prevented by any cryoprotectant studied. Both in replicating and non-replicating cells, freezing and thawing altered the chromatin structure in a cryoprotectant-dependent manner. Interestingly, cells with condensed chromatin, which was strongly stimulated by dimethyl sulfoxide (DMSO) prior to freezing had the highest rate of survival after thawing. Our results will facilitate the design of compounds and procedures to decrease injury to cryopreserved cells. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2019
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