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Effect of ageing on the myosin heavy chain composition of the human sternocleidomastoid muscle
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SYSNO ASEP 0490239 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Effect of ageing on the myosin heavy chain composition of the human sternocleidomastoid muscle Tvůrce(i) Meznaric, M. (SI)
Eržen, I. (SI)
Karen, Petr (FGU-C)
Cvetko, E. (SI)Zdroj.dok. Annals of Anatomy-Anatomischer Anzeiger. - : Elsevier - ISSN 0940-9602
Roč. 216, March (2018), s. 95-99Poč.str. 5 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova human ; ageing ; sternocleidomastoid muscle ; immunohistochemistry ; myosin heavy chain isoforms ; muscle fibre types Vědní obor RIV FP - Ostatní lékařské obory Obor OECD Anatomy and morphology (plant science to be 1.6) CEP LM2015062 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LTC17023 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora FGU-C - RVO:67985823 UT WOS 000430645200012 EID SCOPUS 85040020506 DOI 10.1016/j.aanat.2017.12.001 Anotace The myosin heavy chain (MyHC) composition of ageing limb muscles is transformed into a slower phenotype and expresses fast-twitch fibre type atrophy, presumably due to age-related motor unit remodelling and a change in the patterns of physical activity. It is not known if ageing affects the sternocleidomastoid muscle (SCM) in a similar way. The goal of the study was to analyze the MyHC composition and the size of muscle fibres in the ageing SCM by immunohistochemical methods and quantitative analysis and stereology using our own software for morphometry. We hypothesize that with ageing the MyHC composition of SCM transforms similarly as in ageing limb muscles, but the size of the muscle fibres is less effected as in limb muscles. The study was performed on the autopsy samples of the SCM in 12 older males. The results were compared with those published in our previous study on 15 young adult males. An ageing SCM transforms into a slower MyHC profile: the percentage of slow-twitch fibres is enhanced (numerical proportion 44.6 vs. 31.5%, P < 0.05, area proportion 57.2 vs. 38.4%, P < 0.05). The share of hybrid 2a/2x fibres is diminished (numerical proportion 14.1 vs. 26.8%, P < 0.05), the area proportion of all fast twitch fibres expressing MyHC-2a and 2x is smaller (50.6 vs. 63.5%, P < 0.05), and the area proportion of fibres expressing the fastest myosin isoform MyHC-2x is smaller too (19.0 vs. 34.5%, P < 0.05). The slower phenotype with the preferential reduction of the fibres expressing the fastest MyHC-2x provide circumstantial evidence for: (i) more fast-twitch than slow-twitch motor units being lost, and (ii) reinnervation by the surviving motor units. There appears to be no significant influence on muscle fibre size, which is congruent with relatively unchanged SCM activity during life. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2019
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