Počet záznamů: 1
Methodological issues affecting the study of fish parasites. III. Effect of fish preservation method
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SYSNO ASEP 0488709 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Methodological issues affecting the study of fish parasites. III. Effect of fish preservation method Tvůrce(i) Kvach, Yuriy (UBO-W) ORCID, RID, SAI
Ondračková, Markéta (UBO-W) RID, ORCID, SAI
Janáč, Michal (UBO-W) RID, ORCID, SAI
Jurajda, Pavel (UBO-W) RID, SAI, ORCIDCelkový počet autorů 4 Zdroj.dok. Diseases of Aquatic Organisms. - : Inter-Research - ISSN 0177-5103
Roč. 127, č. 3 (2018), s. 213-224Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova flounder Paralichthys-olivaceus ; Neoheterobothrium-hirame ; community structure ; infection levels ; Baltic sea ; Odontobutidae ; ectoparasites ; Perciformes ; collection ; ecology ; Parasite community ; Preservation methods ; Perca fluviatilis ; Rhodeus amarus ; Methodology ; Parasitological examination Vědní obor RIV GL - Rybářství Obor OECD Fishery CEP GBP505/12/G112 GA ČR - Grantová agentura ČR Institucionální podpora UBO-W - RVO:68081766 UT WOS 000427021400005 EID SCOPUS 85043698364 DOI 10.3354/dao03197 Anotace The aim of this study was to evaluate the influence of preservation method on the results of parasite community studies. Two host species, European perch Perca fluviatilis and European bitterling Rhodeus amarus, were examined for parasites after having been subjected to 4 different storage treatments: freezing, preservation in 4% formaldehyde or 70% ethanol and transportation of live (fresh) fish as a control. Preservation prior to dissection resulted in a loss of information, leading to incomplete quantitative data (all preservation treatments), qualitative data (ethanol and formaldehyde preservation) and a lowered ability to determine parasites to species level based on morphology compared to dissecting fresh fish. Of the more abundant taxa, only crustaceans and acanthocephalans provided relatively even results between treatments. We conclude that preservation media, such as ethanol or formaldehyde, significantly affects the ability to obtain precise parasite community data, hence, we recommend the use of freshly sacrificed fish for parasite community studies whenever possible. Alternatively, freezing may prove acceptable for evaluating parasite community taxonomic composition. Pracoviště Ústav biologie obratlovců Kontakt Hana Slabáková, slabakova@ivb.cz, Tel.: 543 422 524 Rok sběru 2019
Počet záznamů: 1