Počet záznamů: 1
Anaplasma phagocytophilum MSP4 and HSP70 Proteins Are Involved in Interactions with Host Cells during Pathogen Infection
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SYSNO ASEP 0479014 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Anaplasma phagocytophilum MSP4 and HSP70 Proteins Are Involved in Interactions with Host Cells during Pathogen Infection Tvůrce(i) Contreras, M. (ES)
Alberdi, P. (ES)
Mateos-Hernández, L. (ES)
Fernández de Mera, I.G. (ES)
García-Pérez, A. L. (ES)
Vancová, Marie (BC-A) RID, ORCID
Villar, M. (ES)
Ayllón, N. (ES)
Cabezas Cruz, Alejandro (BC-A)
Valdés, James J. (BC-A) RID, ORCID
Stuen, S. (NO)
Gortazar, C. (ES)
de la Fuente, J. (ES)Celkový počet autorů 13 Číslo článku 307 Zdroj.dok. Frontiers in Cellular and Infection Microbiology. - : Frontiers Media - ISSN 2235-2988
Roč. 7, JUL 5 (2017)Poč.str. 16 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova anaplasmosis ; immunology ; hl60 ; tick ; vaccine ; sheep ; Anaplasma phagocytophilum Vědní obor RIV EC - Imunologie Obor OECD Immunology Institucionální podpora BC-A - RVO:60077344 UT WOS 000405138000001 EID SCOPUS 85027553552 DOI 10.3389/fcimb.2017.00307 Anotace Anaplasrna phagocytophilum transmembrane and surface proteins play a role during infection and multiplication in host neutrophils and tick vector cells. Recently, A. phagocytophilum Major surface protein 4 (MSP4) and Heat shock protein 70 (HSP70) were shown to be localized on the bacterial membrane, with a possible role during pathogen infection in ticks. In this study, we hypothesized that A. phagocytophilum MSP4 and HSP70 have similar functions in tick-pathogen and host-pathogen interactions. To address this hypothesis, herein we characterized the role of these bacterial proteins in interaction and infection of vertebrate host cells. The results showed that A. phagocytophilum MSP4 and HSP70 are involved in host-pathogen interactions, with a role for HSP70 during pathogen infection. The analysis of the potential protective capacity of MSP4 and MSP4-HSP70 antigens in immunized sheep showed that MSP4-HSP70 was only partially protective against pathogen infection. This limited protection may be associated with several factors, including the recognition of non-protective epitopes by IgG in immunized lambs. Nevertheless, these antigens may be combined with other candidate protective antigens for the development of vaccines for the control of human and animal granulocytic anaplasmosis. Focusing on the characterization of host protective immune mechanisms and protein-protein interactions at the host-pathogen interface may lead to the discovery and design of new effective protective antigens. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2018
Počet záznamů: 1