Real-time PCR quantification of arbuscular mycorrhizal fungi: does the use of nuclear or mitochondrial markers make a difference?

0478214 - BÚ 2018 RIV DE eng J - Článek v odborném periodiku
Voříšková, Alena - Jansa, J. - Püschel, David - Krüger, Manuela - Cajthaml, T. - Vosátka, Miroslav - Janoušková, Martina
Real-time PCR quantification of arbuscular mycorrhizal fungi: does the use of nuclear or mitochondrial markers make a difference?
Mycorrhiza. Roč. 27, č. 6 (2017), s. 577-585. ISSN 0940-6360. E-ISSN 1432-1890
Grant CEP: GA ČR GA15-05466S
Institucionální podpora: RVO:67985939
Klíčová slova: real-time PCR * quantification * arbuscular mycorrhizal fungi
Obor OECD: Plant sciences, botany
Impakt faktor: 2.778, rok: 2017

Copy numbers of nuclear ribosomal DNA (nrDNA) and mitochondrial DNA (mtDNA) were closely related within all isolates, however, the slopes and intercepts of the linear relationships significantly differed among the isolates. Across all isolates, a large proportion of variance in nrDNA copy numbers was explained by root colonization intensity or contents of AMF-specific PLFA, while variance in mtDNA copy numbers was mainly explained by differences among AMF isolates. Our results suggest that nrDNA is a more suitable marker region than mtDNA for the quantification of multiple AMF taxa as its copy numbers are better related to fungal biomass across taxa than are copy numbers of mtDNA.
Trvalý link: http://hdl.handle.net/11104/0277646