Počet záznamů: 1
Raman and fluorescence microscopy sensing energy-transducing and energy-storing structures in microalgae
- 1.
SYSNO ASEP 0463694 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Raman and fluorescence microscopy sensing energy-transducing and energy-storing structures in microalgae Tvůrce(i) Moudříková, Š. (CZ)
Mojzeš, P. (CZ)
Zachleder, Vilém (MBU-M) ORCID
Pfaff, Ch. (DE)
Behrendt, D. (DE)
Nedbal, L. (CZ)Zdroj.dok. Algal Research-Biomass Biofuels and Bioproducts. - : Elsevier - ISSN 2211-9264
Roč. 16, June (2016), s. 224-232Poč.str. 9 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Biofuels ; Lipids ; Photosynthesis Vědní obor RIV EE - Mikrobiologie, virologie Institucionální podpora MBU-M - RVO:61388971 UT WOS 000375610000026 EID SCOPUS 84961129437 DOI 10.1016/j.algal.2016.03.016 Anotace Chlorophylls and carotenoids are core components of photosynthetic energy transduction in algal cells, whereas starch, lipids, and polyphosphates represent energy reserves. All these biomolecules exhibit characteristic molecular vibrations that were sensed and localized in individual cells of Desmodesmus quadricauda and Chlorella vulgaris by confocal Raman microscopy. In the same cells, fluorescence of chlorophylls and of lipid bodies stained with Nile Red was mapped using the same instrument. In the first of the three consecutive scans, a low-power and short-exposure excitation by a green 532 nm laser was used to map chlorophyll fluorescence. In the second scan, the full power of a 785 nm laser was used for Raman mapping of carotenoids. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2017
Počet záznamů: 1