Počet záznamů: 1
The essential function of the Trypanosoma brucei Trl1 homolog in procyclic cells is maturation of the intron-containing tRNATyr
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SYSNO ASEP 0463449 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The essential function of the Trypanosoma brucei Trl1 homolog in procyclic cells is maturation of the intron-containing tRNATyr Tvůrce(i) Lopes, R.R.S. (BR)
Silveira, G. de O. (BR)
Eitler, R. (BR)
Vidal, R.S. (BR)
Kessler, A. (US)
Hinger, S. (US)
Paris, Zdeněk (BC-A) RID
Alfonzo, J. D. (US)
Polycarpo, C. (BR)Zdroj.dok. RNA. - : Cold Spring Harbor Laboratory Press - ISSN 1355-8382
Roč. 22, č. 8 (2016), s. 1190-1199Poč.str. 10 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Trypanosoma ; tRNA ; tRNA editing ; splicing ; intron Vědní obor RIV EB - Genetika a molekulární biologie CEP GJ15-21450Y GA ČR - Grantová agentura ČR Institucionální podpora BC-A - RVO:60077344 UT WOS 000386679200009 EID SCOPUS 84979686900 DOI 10.1261/rna.056242.116 Anotace Trypanosoma brucei, the etiologic agent of sleeping sickness, encodes a single intron-containing tRNA, tRNA(Tyr), and splicing is essential for its viability. In Archaea and Eukarya, tRNA splicing requires a series of enzymatic steps that begin with intron cleavage by a tRNA-splicing endonuclease and culminates with joining the resulting tRNA exons by a splicing tRNA ligase. Here we explored the function of TbTrl1, the T. brucei homolog of the yeast Trl1 tRNA ligase. We used a combination of RNA interference and molecular biology approaches to show that down-regulation of TbTrl1 expression leads to accumulation of intron-containing tRNA(Tyr) and a concomitant growth arrest at the G1 phase. These defects were efficiently rescued by expression of an "intronless" version of tRNA(Tyr) in the same RNAi cell line. Taken together, these experiments highlight the crucial importance of the TbTrl1 for tRNA(Tyr) maturation and viability, while revealing tRNA splicing as its only essential function Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2017
Počet záznamů: 1