Počet záznamů: 1
Single cell expression analysis of genes with potential mRNA gradient in mouse oocytes.\n\n
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SYSNO ASEP 0459813 Druh ASEP A - Abstrakt Zařazení RIV Záznam nebyl označen do RIV Zařazení RIV Není vybrán druh dokumentu Název Single cell expression analysis of genes with potential mRNA gradient in mouse oocytes.
Tvůrce(i) Dorosh, Andriy (BTO-N) RID
Margaryan, Hasmik (BTO-N) RID
Vodička, Martin (FGU-C) RID, ORCID, SAI
Ergang, Peter (FGU-C) RID, ORCID
Šídová, Monika (BTO-N) RID
Dvořáková-Hortová, Kateřina (BTO-N)Celkový počet autorů 6 Zdroj.dok. Book of abstract XXIInd Symposium of immunology and biology of Reproduction with international participation. - Vestec u Prahy : Biotechnologický ústav, 2016 / Pěknicová Jana ; Kubátová Alena ; Elzeinová Fatima
S. 34-34Poč.str. 1 s. Akce XXIInd Symposium of Immunology and Biology of Reproduction with international participation Datum konání 26.05.2016 - 28.05.2016 Místo konání Třešť Země CZ - Česká republika Typ akce WRD Jazyk dok. eng - angličtina Země vyd. CZ - Česká republika Klíč. slova single cell analysis ; mRNA gradient ; mouse oocytes Vědní obor RIV EB - Genetika a molekulární biologie CEP ED1.1.00/02.0109 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA14-05547S GA ČR - Grantová agentura ČR Institucionální podpora BTO-N - RVO:86652036 Anotace In frogs, there are clearly visible differently pigmented animal and vegetal poles of the egg determined before fertilization and leading to asymmetrical divisions. Mammalian egg does not show any comparable differentiation and it has been generally accepted that even the individual blastomeres in 2-cell and 4-cell embryos are homogenous. However, recent findings suggest that those blastomeres display different gene expression patterns and might already possess some inclinations to specific cell lineages. We therefore raised a question, whether there could be any mRNA or protein gradients in pre-fertilization oocytes similar to a previously described amphibian egg one.
In mammalian eggs, there is a membrane region that is poor in microvilli, cortical granules are absent beneath plasma membrane and sperm cells generally do not bind to this location. This microvilli free region also covers the egg nucleus, and cytoskeleton localization differs markedly to the rest of the cortical space, forming actin –myosin II cortical cap/ring and is considered as animal pole. The purpose of this study was to determine gene products that can be detected at single cell level using qPCR and display gradient like distribution in mature oocytes. We checked expression of 12 selected genes in a pool of 10 oocytes and single mature oocytes. Then, we analysed gene expression in fixed intact oocytes and those undergoing laser capture microdissection procedure (LCMD).
Eventually, we have determined six candidate genes for the study of intracellular spatial gene expression in mature mammalian oocytes by subcellular qPCR and in situ hybridization.
Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2017
Počet záznamů: 1