Počet záznamů: 1
Enzyme-linked electrochemical detection of DNA fragments amplified by PCR in the presence of a biotinylated deoxynucleoside triphosphate using disposable pencil graphite electrodes
- 1.
SYSNO ASEP 0446295 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Enzyme-linked electrochemical detection of DNA fragments amplified by PCR in the presence of a biotinylated deoxynucleoside triphosphate using disposable pencil graphite electrodes Tvůrce(i) Haroniková, Lucia (BFU-R)
Špaček, Jan (BFU-R) ORCID
Plucnara, Medard (BFU-R) ORCID
Horáková, Petra (BFU-R)
Pivoňková, Hana (BFU-R) ORCID
Havran, Luděk (BFU-R) RID, ORCID
Erdem, A. (TR)
Fojta, Miroslav (BFU-R) RID, ORCIDCelkový počet autorů 8 Zdroj.dok. Monatshefte fur Chemie. - : Springer - ISSN 0026-9247
Roč. 146, č. 5 (2015), s. 849-855Poč.str. 7 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. AT - Rakousko Klíč. slova POLYMERASE-CHAIN-REACTION ; V-LEIDEN MUTATION ; SENSITIVE DETECTION Vědní obor RIV BO - Biofyzika CEP GAP206/11/1638 GA ČR - Grantová agentura ČR GPP206/11/P739 GA ČR - Grantová agentura ČR Institucionální podpora BFU-R - RVO:68081707 UT WOS 000353219700015 DOI 10.1007/s00706-015-1436-5 Anotace In this report, we present a simple electrochemical detection protocol for the detection of specific PCR-amplified DNA fragments, based on incorporation of biotin tags into DNA amplicons during PCR run in the presence of a biotinylated nucleoside triphosphate. For detection, an enzyme-linked electrochemical system involving streptavidin-alkaline phosphatase conjugate attached to the biotinylated DNA, adsorbed at the surface of a disposable pencil graphite electrode, is used. The enzyme converts an inactive indicator, 1-naphthyl phosphate, into electrochemically oxidizable indicator 1-naphthol that is subsequently detected. Excellent selectivity of this fast, facile, and inexpensive analysis not requiring any sophisticated electrode modification and its applicability for off-line monitoring of DNA amplification is demonstrated. Applications of the technique include detection of the presence of specific nucleotide sequences in biological samples, such as sequences related to pathogenic microorganism or transgenes. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2016
Počet záznamů: 1