Počet záznamů: 1
Macroporous 2-hydroxyethyl methacrylate hydrogels of dual porosity for cell cultivation: morphology, swelling, permeability, and mechanical behavior
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SYSNO ASEP 0435661 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Macroporous 2-hydroxyethyl methacrylate hydrogels of dual porosity for cell cultivation: morphology, swelling, permeability, and mechanical behavior Tvůrce(i) Přádný, Martin (UMCH-V) RID
Dušková-Smrčková, Miroslava (UMCH-V) RID
Dušek, Karel (UMCH-V) RID, ORCID
Janoušková, Olga (UMCH-V) RID, SAI, ORCID
Sadakbayeva, Zhansaya (UMCH-V) RID
Šlouf, Miroslav (UMCH-V) RID, ORCID
Michálek, Jiří (UMCH-V) RID, ORCIDZdroj.dok. Journal of Polymer Research. - : Springer - ISSN 1022-9760
Roč. 21, č. 11 (2014), 579_1-579_12Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. TW - Taiwan - Čína Klíč. slova hydrogel ; 2-hydroxyethyl methacrylate ; porosity Vědní obor RIV CD - Makromolekulární chemie CEP GAP108/12/1538 GA ČR - Grantová agentura ČR Institucionální podpora UMCH-V - RVO:61389013 UT WOS 000351130200001 EID SCOPUS 84911936639 DOI 10.1007/s10965-014-0579-0 Anotace Macroporous hydrogels of dual porosity based on cross-linked poly(2-hydroxyethyl methacrylate) (pHEMA) were prepared, and their swelling, mechanical responses, and hydraulic permeability, as well as cell adhesion and proliferation, were examined on gel-based scaffolds. Large pores on the order of tens and hundreds of microns were generated by adding a solid porogen (fractionated particles of sodium chloride) to the polymerization mixture, which was subsequently washed out of the gel. Small pores on the order of a few microns were formed by the reaction-induced phase separation mechanism caused by addition of a diluent (1-dodecanol), which is a poor solvent for pHEMA. Morphological studies using light and electron microscopy techniques revealed that the large pores were embedded in the pHEMA matrix containing small fused spherical pores. Mechanical behavior of the dual-porosity hydrogels characterized by oscillatory shear measurements revealed that despite very high pore volume, the hydrogels were sufficiently stiff and self-supportive. The cell cultivation pilot experiment showed that the morphology (dual porosity) demonstrated marked effects on the promotion of cell adhesion, growth, and proliferation, not only on the gel surface but in the large pores within the gel bulk as well. Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2015
Počet záznamů: 1