Počet záznamů: 1
The isolation and characterization of lipopolysaccharides from Microcystis aeruginosa, a prominent toxic water bloom forming cyanobacteria
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SYSNO ASEP 0424726 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The isolation and characterization of lipopolysaccharides from Microcystis aeruginosa, a prominent toxic water bloom forming cyanobacteria Tvůrce(i) Blahová, L. (CZ)
Adamovský, O. (CZ)
Kubala, Lukáš (BFU-R) RID, ORCID
Šindlerová, Lenka (BFU-R) RID, ORCID
Zounková, R. (CZ)
Blaha, L. (CZ)Celkový počet autorů 6 Zdroj.dok. Toxicon. - : Elsevier - ISSN 0041-0101
Roč. 76, DEC (2013), s. 187-196Poč.str. 10 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova CYLINDROSPERMOPSIS-RACIBORSKII ; ESCHERICHIA-COLI ; DANIO-RERIO Vědní obor RIV BO - Biofyzika Institucionální podpora BFU-R - RVO:68081707 UT WOS 000328658600023 EID SCOPUS 84886285044 DOI 10.1016/j.toxicon.2013.10.011 Anotace Massive toxic blooms of cyanobacteria represent a major threat to water supplies worldwide, yet serious gaps exist in understanding their complex toxic effects, including the role of lipopolysaccharides (LPS). The present comparative study focused on the levels and biological activities of LPS isolated from Microcystis aeruginosa, which is one of the most globally distributed toxic species. Using hot phenol extraction, LPS was isolated from 3 laboratory cultures and 11 natural water blooms. It formed 0.2-0.7% of the original dry biomass of the cyanobacteria, based on gravimetry. Additional analyses by commercial anti-LPS ELISA were correlated with gravimetry but showed concentrations that were about 7-times lower, which indicated either impurities in isolated LPS or the poor cross-reactivity of the antibodies used. LPS isolates from M. aeruginosa were potent pyrogens in the traditional Limulus amebocyte lysate (LAL)-test, but comparison with the PyroGene test demonstrated the limited selectivity of LAL with several interferences. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2014
Počet záznamů: 1