Počet záznamů: 1
Compact surface plasmon-enhanced fluorescence biochip
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SYSNO ASEP 0396519 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Compact surface plasmon-enhanced fluorescence biochip Tvůrce(i) Toma, K. (AT)
Vala, Milan (URE-Y) RID
Adam, Pavel (URE-Y) RID
Homola, Jiří (URE-Y) RID
Knoll, W. (AT)
Dostálek, J. (AT)Celkový počet autorů 6 Zdroj.dok. Optics Express. - : Optical Society of America - ISSN 1094-4087
Roč. 21, č. 8 (2013), s. 10121-10132Poč.str. 12 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Surface plasmons ; Diffraction gratings ; Biological sensing and sensors Vědní obor RIV BH - Optika, masery a lasery CEP GBP205/12/G118 GA ČR - Grantová agentura ČR Institucionální podpora URE-Y - RVO:67985882 UT WOS 000318151600088 DOI 10.1364/OE.21.010121 Anotace A new concept of compact biochip for surface plasmon-enhanced fluorescence assays is reported. It takes advantage of the amplification of fluorescence signal through the coupling of fluorophore labels with confined and strongly enhanced field intensity of surface plasmons. In order to efficiently excite and collect the emitted fluorescence light via surface plasmons on a metallic sensor surface, (reverse) Kretschmann configuration is combined with diffractive optical elements embedded on the chip surface. These include a concentric relief grating for the imaging of highly directional surface plasmon-coupled emission to a detector. Additional linear grating is used for the generating of surface plasmons at the excitation wavelength on the sensor surface in order to increase the fluorescence excitation rate. The reported approach offers the increased intensity of fluorescence signal, reduced background, and compatibility with nanoimprint lithography for cost-effective preparation of sensor chip. The presented approach was implemented for biosensing in a model immunoassay experiment in which the limit of detection of 11 pM was achieved. Pracoviště Ústav fotoniky a elektroniky Kontakt Petr Vacek, vacek@ufe.cz, Tel.: 266 773 413, 266 773 438, 266 773 488 Rok sběru 2014
Počet záznamů: 1