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The transmembrane region is responsible for targeting of adaptor protein LAX into "heavy rafts''
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SYSNO ASEP 0387633 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The transmembrane region is responsible for targeting of adaptor protein LAX into "heavy rafts'' Tvůrce(i) Hrdinka, Matouš (UMG-J)
Otáhal, Pavel (UMG-J) RID
Hořejší, Václav (UMG-J) RIDZdroj.dok. PLoS ONE. - : Public Library of Science - ISSN 1932-6203
Roč. 7, č. 5 (2012), e36330Poč.str. 6 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova LAX ; transmembrane domain ; DRM Vědní obor RIV EB - Genetika a molekulární biologie CEP GEMEM/09/E011 GA ČR - Grantová agentura ČR 1M0506 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000305342300011 DOI 10.1371/journal.pone.0036330 Anotace The importance of membrane compartmentalization into specific membrane microdomains has been shown in many biological processes such as immunoreceptor signaling, membrane trafficking, pathogen infection, and tumor progression. Microdomains like lipid rafts, caveolae and tetraspanin enriched microdomains are relatively resistant to solubilization by some detergents. Large detergent-resistant membrane fragments (DRMs) resulting from such membrane solubilization can be conveniently isolated by density gradient ultracentrifugation or gel filtration. Recently, we described a novel type of raft-like membrane microdomains producing, upon detergent Brij98 solubilization, “heavy DRMs” and containing a number of functionally relevant proteins. Transmembrane adaptor protein LAX is a typical “heavy raft” protein. The present study was designed to identify the molecular determinants targeting LAX-derived constructs to heavy rafts. We identified the transmembrane domain of LAX as a first motif targeting transmembrane protein constructs to detergent-resistant heavy rafts, a novel type of membrane microdomains containing a number of physiologically important proteins. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2013
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