Počet záznamů: 1
Primary enamel knot cell death in Apaf-1 and caspase-9 deficient mice
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SYSNO ASEP 0082446 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Ostatní články Název Primary enamel knot cell death in Apaf-1 and caspase-9 deficient mice Překlad názvu Buněčná smrt v primárním sklovinném uzlu u Apaf-1 a kaspáza-9 deficientních myší Tvůrce(i) Šetková, Jana (UZFG-Y)
Matalová, Eva (UZFG-Y) RID
Sharpe, P. T. (GB)
Míšek, Ivan (UZFG-Y)
Tucker, A. S. (GB)Zdroj.dok. Archives of Oral Biology. - : Elsevier - ISSN 0003-9969
Roč. 52, 1 (2007), s. 15-19Poč.str. 5 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova dental apoptosis ; apoptosome ; apaf-1 knockout ; caspase-9 knockout Vědní obor RIV FF - ORL, oftalmologie, stomatologie CEP KJB500450503 GA AV ČR - Akademie věd GA304/04/0101 GA ČR - Grantová agentura ČR OC B23.001 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy CEZ AV0Z50450515 - UZFG-Y (2005-2011) Anotace During molar development, apoptosis occurs in a well-characterized pattern suggesting several roles for cell death in odontogenesis. However, molecular mechanisms of dental apoptosis are only poorly understood. In this study, Apaf-1 and caspase-9 knockouts were used to uncover the engagement of these members of the apoptotic machinery during early tooth development, concentrating primarily on their function in the apoptotic elimination of primary enamel knot cells. Molar tooth germ morphology, proliferation and apoptosis were investigated on frontal histological sections of murine heads at embryonic days (ED) 15.5 - the stage when the primary enamel knot is eliminated apoptotically. In molar tooth germs of both knockouts, no apoptosis was observed according to morphological (haematoxylin-eosin) as well as biochemical criteria (TUNEL). Morphology of the mutant tooth germs, however, was not changed. Additionally, knockout mice showed no changes in proliferation compared to wild type mice. According to our findings on knockout embryos, Apaf-1 and caspase-9 are involved in apoptosis during tooth development; however, they seem dispensable and not necessary for proper tooth shaping. Compensatory or other mechanisms of cell death may act to eliminate the primary enamel knot cells in the absence of Apaf-1 and caspase-9. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2007
Počet záznamů: 1