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Titanium-Doped Diamond-like Carbon Layers as a Promising Coating for Joint Replacements Supporting Osteogenic Differentiation of Mesenchymal Stem Cells
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SYSNO ASEP 0583915 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Titanium-Doped Diamond-like Carbon Layers as a Promising Coating for Joint Replacements Supporting Osteogenic Differentiation of Mesenchymal Stem Cells Tvůrce(i) Trávníčková, Martina (FGU-C) RID, ORCID, SAI
Filová, Elena (FGU-C) RID, ORCID
Slepička, P. (CZ)
Kasálková-Slepičková, N. (CZ)
Kocourek, Tomáš (FZU-D) RID, ORCID, SAI
Žaloudková, Margit (USMH-B) RID
Suchý, Tomáš (USMH-B) RID, ORCID, SAI
Bačáková, Lucie (FGU-C) RID, ORCIDČíslo článku 2837 Zdroj.dok. International Journal of Molecular Sciences. - : MDPI - ISSN 1661-6596
Roč. 25, č. 5 (2024)Poč.str. 27 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova titanium ; diamond-like carbon layer (DLC) ; biocompatibility ; osteogenic differentiation ; adipose tissue-derived stem cells (ADSCs) ; bone marrow mesenchymal stem cells (bmMSCs) Obor OECD Orthopaedics Vědní obor RIV – spolupráce Fyzikální ústav - Koroze a povrchové úpravy materiálů CEP GA20-01570S GA ČR - Grantová agentura ČR EH22_008/0004562 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora FGU-C - RVO:67985823 ; FZU-D - RVO:68378271 ; USMH-B - RVO:67985891 UT WOS 001182815400001 EID SCOPUS 85187686810 DOI 10.3390/ijms25052837 Anotace Diamond-like carbon (DLC) layers are known for their high corrosion and wear resistance, low friction, and high biocompatibility. However, it is often necessary to dope DLC layers with additional chemical elements to strengthen their adhesion to the substrate. Ti-DLC layers (doped with 0.4, 2.1, 3.7, 6.6, and 12.8 at.% of Ti) were prepared by dual pulsed laser deposition, and pure DLC, glass, and polystyrene (PS) were used as controls. In vitro cell–material interactions were investigated with an emphasis on cell adhesion, proliferation, and osteogenic differentiation. We observed slightly increasing roughness and contact angle and decreasing surface free energy on Ti-DLC layers with increasing Ti content. Three-week biological experiments were performed using adipose tissue-derived stem cells (ADSCs) and bone marrow mesenchymal stem cells (bmMSCs) in vitro. The cell proliferation activity was similar or slightly higher on the Ti-doped materials than on glass and PS. Osteogenic cell differentiation on all materials was proved by collagen and osteocalcin production, ALP activity, and Ca deposition. The bmMSCs exhibited greater initial proliferation potential and an earlier onset of osteogenic differentiation than the ADSCs. The ADSCs showed a slightly higher formation of focal adhesions, higher metabolic activity, and Ca deposition with increasing Ti content. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2025 Elektronická adresa https://www.mdpi.com/1422-0067/25/5/2837
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