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Electrochemical flow-through biosensors based on microfiber enzymatic filter discs placed at printed electrodes.
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SYSNO ASEP 0583598 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Electrochemical flow-through biosensors based on microfiber enzymatic filter discs placed at printed electrodes. Tvůrce(i) Josypčuk, Bohdan (UFCH-W) RID, SAI, ORCID
Tvorynska, Sofiia (UFCH-W) ORCID, RIDČíslo článku 108663 Zdroj.dok. Bioelectrochemistry. - : Elsevier - ISSN 1567-5394
Roč. 157, JUN 2024 (2024)Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova biosensors ; electrochemistry ; enzymatic discs Obor OECD Physical chemistry CEP GA23-07292S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora UFCH-W - RVO:61388955 UT WOS 001183464500001 EID SCOPUS 85185194393 DOI 10.1016/j.bioelechem.2024.108663 Anotace A new type of electrochemical biosensors in a flow injection system with printed electrodes were developed and tested. A filter disc (7mm diameter) with immobilized enzyme was placed at the printed electrode. This conception combines the advantages of biosensors with a bioreceptor at the electrode surface and systems with spatially separated enzymatic and detection parts. Filters of different composition (glass, quartz, and cellulose), thickness, porosity, and ways of binding enzyme to their surface were tested. Only covalent bonds throughout a filter-aminosilane-glutaraldehyde-enzyme chain ensured a long-time and reproducible biosensor response. The developed method of biosensor preparation has been successfully applied to enzymes glucose oxidase, laccase and choline oxidase. The dependences of peak current on detection potential, flow rate, injection volume, analyte concentration as well as biosensor lifetime and reproducibility were investigated for glucose oxidase biosensor. The sensitivity of measurements was two or more times higher than that of biosensor with a mini-reactor filled by powder with immobilized enzyme. The developed biosensor with laccase was tested by determining dopamine in the pharmaceutical infusion product Tensamin. Results of the analysis (40.0±0.7mgmL-1, SD=0.8mgmL-1, RSD=1.85%, N=11) show a good agreement with the manufacturer's declared value. Pracoviště Ústav fyzikální chemie J.Heyrovského Kontakt Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Rok sběru 2025 Elektronická adresa https://hdl.handle.net/11104/0351603
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