Počet záznamů: 1
Conjugation of microbial-derived gold nanoparticles to different types of nucleic acids: evaluation of transfection efficiency.
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SYSNO ASEP 0579986 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Conjugation of microbial-derived gold nanoparticles to different types of nucleic acids: evaluation of transfection efficiency. Tvůrce(i) Pourali, Parastoo (MBU-M)
Dzmitruk, Volha (BTO-N)
Benada, Oldřich (MBU-M) ORCID, RID
Svoboda, Milan (UIACH-O) RID, ORCID
Benson, Veronika (MBU-M) RID, ORCIDZdroj.dok. Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 13, September 6 (2023), s. 14669Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova gold nanoparticles ; capping agent ; biosynthesis ; nucleic acid transfection ; drug carriers Obor OECD Microbiology CEP EH22_010/0002357 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2018127 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Výzkumná infrastruktura CIISB II - 90127 - Masarykova univerzita Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 ; BTO-N - RVO:86652036 ; UIACH-O - RVO:68081715 UT WOS 001138051400001 EID SCOPUS 85170103294 DOI 10.1038/s41598-023-41567-7 Anotace In this study, gold nanoparticles produced by eukaryotic cell waste (AuNP), were analyzed as a transfection tool. AuNP were produced by Fusarium oxysporum and analyzed by spectrophotometry, transmission electron microscopy (TEM), scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy (EDS). Fourier transform infrared spectroscopy (FTIR) and dynamic light scattering (DLS) were used before and after conjugation with different nucleic acid (NA) types. Graphite furnace atomic absorption spectroscopy (GF-AAS) was used to determine the AuNP concentration. Conjugation was detected by electrophoresis. Confocal microscopy and quantitative real-time PCR (qPCR) were used to assess transfection. TEM, SEM, and EDS showed 25nm AuNP with round shape. The amount of AuNP was 3.75±0.2g/L and FTIR proved conjugation of all NA types to AuNP. All the samples had a negative charge of36 to46mV. Confocal microscopy confirmed internalization of the ssRNA-AuNP into eukaryotic cells and qPCR confirmed release and activity of carried RNA. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2024 Elektronická adresa https://www.nature.com/articles/s41598-023-41567-7
Počet záznamů: 1