Počet záznamů: 1
Dimerisation of the Yeast K+Translocation Protein Trk1 Depends on the K+Concentration
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SYSNO ASEP 0578992 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Dimerisation of the Yeast K+Translocation Protein Trk1 Depends on the K+Concentration Tvůrce(i) Kulik, Natalia (MBU-M) ORCID
Kale, Deepika (MBU-M) ORCID, RID
Spurná, Karin (MBU-M)
Shamayeva, Katsiaryna (MBU-M) ORCID
Hauser, F. (AT)
Milic, S. (AT)
Janout, H. (AT)
Zayats, Vasilina (MBU-M) ORCID
Jacak, J. (AT)
Ludwig, Jost (MBU-M) ORCIDČíslo článku 398 Zdroj.dok. International Journal of Molecular Sciences. - : MDPI
Roč. 24, č. 1 (2023)Poč.str. 16 s. Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova affinity potassium-transport ; saccharomyces-cerevisiae ; crystal-structure ; membrane-protein ; gene disruption ; channel ; strains ; network ; encodes ; systems ; K+ translocation ; Saccharomyces cerevisiae ; bimolecular fluorescence complementation ; dimerisation ; molecular modelling ; MD simulation Obor OECD Microbiology CEP GA16-19221S GA ČR - Grantová agentura ČR LM2015055 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy 8J22AT012 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 000909320300001 EID SCOPUS 85145987316 DOI 10.3390/ijms24010398 Anotace In baker's yeast (Saccharomyces cerevisiae), Trk1, a member of the superfamily of K-transporters (SKT), is the main K+ uptake system under conditions when its concentration in the environment is low. Structurally, Trk1 is made up of four domains, each similar and homologous to a K-channel alpha subunit. Because most K-channels are proteins containing four channel-building alpha subunits, Trk1 could be functional as a monomer. However, related SKT proteins TrkH and KtrB were crystallised as dimers, and for Trk1, a tetrameric arrangement has been proposed based on molecular modelling. Here, based on Bimolecular Fluorescence Complementation experiments and single-molecule fluorescence microscopy combined with molecular modelling, we provide evidence that Trk1 can exist in the yeast plasma membrane as a monomer as well as a dimer. The association of monomers to dimers is regulated by the K+ concentration. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2024 Elektronická adresa https://www.mdpi.com/1422-0067/24/1/398
Počet záznamů: 1