Počet záznamů: 1
Conserved satellite DNA motif and lack of interstitial telomeric sites in highly rearranged African Nothobranchius killifish karyotypes
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SYSNO ASEP 0576446 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Conserved satellite DNA motif and lack of interstitial telomeric sites in highly rearranged African Nothobranchius killifish karyotypes Tvůrce(i) Lukšíková, Karolína (UZFG-Y)
Pavlica, Tomáš (UZFG-Y)
Altmanová, Marie (UZFG-Y) ORCID
Štundlová, Jana (UZFG-Y)
Pelikánová, Šárka (UZFG-Y)
Simanovsky, S. A. (RU)
Krysanov, E. Y. (RU)
Jankásek, Marek (UZFG-Y)
Hiřman, Matyáš (UZFG-Y)
Reichard, Martin (UBO-W) RID, ORCID, SAI, SAI
Ráb, Petr (UZFG-Y) RID, ORCID
Sember, Alexandr (UZFG-Y) RID, ORCIDZdroj.dok. Journal of Fish Biology. - : Wiley - ISSN 0022-1112
Roč. 103, č. 6 (2023), s. 1501-1514Poč.str. 14 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova chromosome fusion ; chromosome polymorphism ; FISH Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Genetics and heredity (medical genetics to be 3) CEP GJ19-22346Y GA ČR - Grantová agentura ČR EF15_003/0000460 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UZFG-Y - RVO:67985904 ; UBO-W - RVO:68081766 UT WOS 001070903200001 EID SCOPUS 85171591280 DOI 10.1111/jfb.15550 Anotace Using African annual killifishes of the genus Nothobranchius from temporary savannah pools with rapid karyotype and sex chromosome evolution, we analysed the chromosomal distribution of telomeric (TTAGGG)(n) repeat and Nfu-SatC satellite DNA (satDNA, isolated from Nothobranchius furzeri) in 15 species across the Nothobranchius killifish phylogeny, and with Fundulosoma thierryi as an out-group. Our fluorescence in situ hybridization experiments revealed that all analysed taxa share the presence of Nfu-SatC repeat but with diverse organization and distribution on chromosomes. Nfu-SatC landscape was similar in conspecific populations of Nothobranchius guentheri and Nothobranchius melanospilus but slightly-to-moderately differed between populations of Nothobranchius pienaari, and between closely related Nothobranchius kuhntae and Nothobranchius orthonotus. Inter-individual variability in Nfu-SatC patterns was found in N. orthonotus and Nothobranchius krysanovi. We revealed mostly no sex-linked patterns of studied repetitive DNA distribution. Only in Nothobranchius brieni, possessing multiple sex chromosomes, Nfu-SatC repeat occupied a substantial portion of the neo-Y chromosome, similarly as formerly found in the XY sex chromosome system of turquoise killifish N. furzeri and its sister species Nothobranchius kadleci-representatives not closely related to N. brieni. All studied species further shared patterns of expected telomeric repeats at the ends of all chromosomes and no additional interstitial telomeric sites. In summary, we revealed (i) the presence of conserved satDNA class in Nothobranchius clades (a rare pattern among ray-finned fishes), (ii) independent trajectories of Nothobranchius sex chromosome differentiation, with recurrent and convergent accumulation of Nfu-SatC on the Y chromosome in some species, and (iii) genus-wide shared tendency to loss of telomeric repeats during interchromosomal rearrangements. Collectively, our findings advance our understanding of genome structure, mechanisms of karyotype reshuffling, and sex chromosome differentiation in Nothobranchius killifishes from the genus-wide perspective. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2024 Elektronická adresa https://onlinelibrary.wiley.com/doi/10.1111/jfb.15550
Počet záznamů: 1