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Detection of metallothionein as a melanoma marker by LA-ICP-MS combined with sample pretreatment by using magnetic particles coated with an imprinted polymeric layer
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SYSNO ASEP 0574401 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Detection of metallothionein as a melanoma marker by LA-ICP-MS combined with sample pretreatment by using magnetic particles coated with an imprinted polymeric layer Tvůrce(i) Veverková, K. (CZ)
Pavelicová, K. (CZ)
Vlčnovská, M. (CZ)
Vejvodová, M. (CZ)
Horák, Vratislav (UZFG-Y) RID, ORCID
Kanický, V. (CZ)
Adam, V. (CZ)
Vaculovič, T. (CZ)
Vaculovičová, M. (CZ)Zdroj.dok. Journal of Analytical Atomic Spectrometry. - : Royal Society of Chemistry - ISSN 0267-9477
Roč. 38, č. 8 (2023), s. 1618-1625Poč.str. 8 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova melanoma ; metallothionein Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Oncology CEP LQ1601 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA20-02203S GA ČR - Grantová agentura ČR EF16_019/0000785 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Omezený přístup Institucionální podpora UZFG-Y - RVO:67985904 UT WOS 001022916300001 EID SCOPUS 85165440787 DOI 10.1039/d3ja00065f Anotace Effective detection and sensitive quantification of disease markers enable a better understanding of processes leading to disease development. Therefore, we present an approach that combines the excellent selective recognition capabilities of molecularly imprinted polymers (MIPs) and sensitive elemental detection using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Dopamine was used as a functional monomer, and metallothionein (MT), a metal-containing protein (Zn and Cd), was the targeted tumor marker. Iron oxide magnetic particles were exploited as a carrier of the polymeric layer. Due to the agglomeration of the particles in the presence of an external magnet, the prepared molecularly imprinted polymers (Mag-MIPs(MT)) were capable of isolating MT molecules from a tissue sample. A sandwich immunoassay with antibodies labeled by using silver nanoparticles was used to confirm the binding of MT to Mag-MIPs(MT). The calculated efficiency of isolation from a standard MT solution (73.5 & mu,M) was 63.3% (46.5 & mu,M). Casein was used to verify the selectivity of the prepared Mag-MIPs. The prepared particles were used for sensitive quantification of MT in melanoma and healthy skin of the melanoma-bearing Libechov minipig (MeLiM), which confirms that this method is applicable for the investigation of the tumor microenvironment. We found a significantly higher content of Zn in tumor tissue than in healthy skin. Pracoviště Ústav živočišné fyziologie a genetiky Kontakt Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Rok sběru 2024 Elektronická adresa https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=5452124316
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