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Aspergillus niger as a cell factory for the production of pyomelanin, a molecule with UV-C radiation shielding activity
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SYSNO ASEP 0574278 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Aspergillus niger as a cell factory for the production of pyomelanin, a molecule with UV-C radiation shielding activity Tvůrce(i) Koch, S. M. (DE)
Freidank-Pohl, C. (DE)
Siontas, O. (DE)
Cortesao, M. (DE)
Mota, A. (DE)
Runzheimer, K. (DE)
Jung, S. (DE)
Rebrošová, K. (CZ)
Šiler, Martin (UPT-D) RID, ORCID, SAI
Moeller, R. (DE)
Meyer, V. (DE)Celkový počet autorů 11 Číslo článku 1233740 Zdroj.dok. Frontiers in Microbiology. - : Frontiers Research Foundation - ISSN 1664-302X
Roč. 14, 20 July (2023)Poč.str. 15 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova Aspergillus niger ; melanin ; pyomelanin ; radioprotection ; cosmic radiation ; space exploration Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP NU21-05-00341 GA MZd - Ministerstvo zdravotnictví Způsob publikování Open access Institucionální podpora UPT-D - RVO:68081731 UT WOS 001041737100001 EID SCOPUS 85166760553 DOI 10.3389/fmicb.2023.1233740 Anotace Melanins are complex pigments with various biological functions and potential applications in space exploration and biomedicine due to their radioprotective properties. Aspergillus niger, a fungus known for its high radiation resistance, is widely used in biotechnology and a candidate for melanin production. In this study, we investigated the production of fungal pyomelanin (PyoFun) in A. niger by inducing overproduction of the pigment using L-tyrosine in a recombinant ΔhmgA mutant strain (OS4.3). The PyoFun pigment was characterized using three spectroscopic methods, and its antioxidant properties were assessed using a DPPH-assay. Additionally, we evaluated the protective effect of PyoFun against non-ionizing radiation (monochromatic UV-C) and compared its efficacy to a synthetically produced control pyomelanin (PyoSyn). The results confirmed successful production of PyoFun in A. niger through inducible overproduction. Characterization using spectroscopic methods confirmed the presence of PyoFun, and the DPPH-assay demonstrated its strong antioxidant properties. Moreover, PyoFun exhibited a highly protective effect against radiation-induced stress, surpassing the protection provided by PyoSyn. The findings of this study suggest that PyoFun has significant potential as a biological shield against harmful radiation. Notably, PyoFun is synthesized extracellularly, differing it from other fungal melanins (such as L-DOPA- or DHN-melanin) that require cell lysis for pigment purification. This characteristic makes PyoFun a valuable resource for biotechnology, biomedicine, and the space industry. However, further research is needed to evaluate its protective effect in a dried form and against ionizing radiation. Pracoviště Ústav přístrojové techniky Kontakt Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Rok sběru 2024 Elektronická adresa https://www.frontiersin.org/articles/10.3389/fmicb.2023.1233740/full
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