Počet záznamů: 1
Accumulation of Cyanobacterial Photosystem II Containing the 'Rogue' D1 Subunit Is Controlled by FtsH Protease and Synthesis of the Standard D1 Protein
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SYSNO ASEP 0573481 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Accumulation of Cyanobacterial Photosystem II Containing the 'Rogue' D1 Subunit Is Controlled by FtsH Protease and Synthesis of the Standard D1 Protein Tvůrce(i) Masuda, Takako (MBU-M) ORCID
Bečková, Martina (MBU-M) RID
Turóczy, Zoltán (MBU-M)
Pilný, Jan (MBU-M) ORCID
Sobotka, Roman (MBU-M) RID, ORCID
Trinugroho, J. P. (GB)
Nixon, P. J. (GB)
Prášil, Ondřej (MBU-M) RID, ORCID
Komenda, Josef (MBU-M) RID, ORCIDZdroj.dok. Plant and Cell Physiology. - : Oxford University Press - ISSN 0032-0781
Roč. 64, č. 6 (2023), s. 660-673Poč.str. 14 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova Chlorophyll biosynthesis ; Crocosphaera watsonii ; Photosystem II ; Rogue D1 ; Synechocystis Obor OECD Plant sciences, botany CEP GA20-17627S GA ČR - Grantová agentura ČR GX19-29225X GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora MBU-M - RVO:61388971 UT WOS 000982799700001 EID SCOPUS 85162739112 DOI 10.1093/pcp/pcad027 Anotace Unicellular diazotrophic cyanobacteria contribute significantly to the photosynthetic productivity of the ocean and the fixation of molecular nitrogen, with photosynthesis occurring during the day and nitrogen fixation during the night. In species like Crocosphaera watsonii WH8501, the decline in photosynthetic activity in the night is accompanied by the disassembly of oxygen-evolving photosystem II (PSII) complexes. Moreover, in the second half of the night phase, a small amount of rogue D1 (rD1), which is related to the standard form of the D1 subunit found in oxygen-evolving PSII, but of unknown function, accumulates but is quickly degraded at the start of the light phase. We show here that the removal of rD1 is independent of the rD1 transcript level, thylakoid redox state and trans-thylakoid pH but requires light and active protein synthesis. We also found that the maximal level of rD1 positively correlates with the maximal level of chlorophyll (Chl) biosynthesis precursors and enzymes, which suggests a possible role for rogue PSII (rPSII) in the activation of Chl biosynthesis just before or upon the onset of light, when new photosystems are synthesized. By studying strains of Synechocystis PCC 6803 expressing Crocosphaera rD1, we found that the accumulation of rD1 is controlled by the light-dependent synthesis of the standard D1 protein, which triggers the fast FtsH2-dependent degradation of rD1. Affinity purification of FLAG-tagged rD1 unequivocally demonstrated the incorporation of rD1 into a non-oxygen-evolving PSII complex, which we term rPSII. The complex lacks the extrinsic proteins stabilizing the oxygen-evolving Mn4CaO5 cluster but contains the Psb27 and Psb28-1 assembly factors. Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2024 Elektronická adresa https://academic.oup.com/pcp/article/64/6/660/7091590?login=true
Počet záznamů: 1