Počet záznamů: 1
ISL1 controls pancreatic alpha cell fate and beta cell maturation
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SYSNO ASEP 0571545 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název ISL1 controls pancreatic alpha cell fate and beta cell maturation Tvůrce(i) Bohuslavová, Romana (BTO-N) RID
Fabriciová, Valeria (BTO-N)
Lebron-Mora, Laura (BTO-N)
Malfatti, Jessica (BTO-N)
Smolík, Ondřej (BTO-N)
Valihrach, Lukáš (BTO-N) RID, ORCID
Benešová, Šárka (BTO-N)
Žucha, Daniel (BTO-N)
Berková, Z. (CZ)
Saudek, F. (CZ)
Evans, S. M. (US)
Pavlínková, Gabriela (BTO-N) RID, ORCIDCelkový počet autorů 12 Číslo článku 53 Zdroj.dok. Cell and Bioscience. - : BioMed Central
Roč. 13, č. 1 (2023)Poč.str. 24 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Pancreatic endocrine cells ; Epigenetic histone modification ; Transcriptome ; Pancreas development Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Biochemistry and molecular biology CEP GA19-07378S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora BTO-N - RVO:86652036 UT WOS 000948348700001 EID SCOPUS 85149989362 DOI 10.1186/s13578-023-01003-9 Anotace BackgroundGlucose homeostasis is dependent on functional pancreatic alpha and ss cells. The mechanisms underlying the generation and maturation of these endocrine cells remain unclear.ResultsWe unravel the molecular mode of action of ISL1 in controlling alpha cell fate and the formation of functional ss cells in the pancreas. By combining transgenic mouse models, transcriptomic and epigenomic profiling, we uncover that elimination of Isl1 results in a diabetic phenotype with a complete loss of alpha cells, disrupted pancreatic islet architecture, downregulation of key ss-cell regulators and maturation markers of ss cells, and an enrichment in an intermediate endocrine progenitor transcriptomic profile.ConclusionsMechanistically, apart from the altered transcriptome of pancreatic endocrine cells, Isl1 elimination results in altered silencing H3K27me3 histone modifications in the promoter regions of genes that are essential for endocrine cell differentiation. Our results thus show that ISL1 transcriptionally and epigenetically controls alpha cell fate competence, and ss cell maturation, suggesting that ISL1 is a critical component for generating functional alpha and ss cells. Pracoviště Biotechnologický ústav Kontakt Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Rok sběru 2024 Elektronická adresa https://cellandbioscience.biomedcentral.com/articles/10.1186/s13578-023-01003-9
Počet záznamů: 1