Počet záznamů: 1
Nanoscale membrane curvature sorts lipid phases and alters lipid diffusion
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SYSNO ASEP 0570657 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Nanoscale membrane curvature sorts lipid phases and alters lipid diffusion Tvůrce(i) Woodward, X. (US)
Javanainen, Matti (UOCHB-X) RID, ORCID
Fábián, Balázs (UOCHB-X) ORCID
Kelly, C. V. (US)Zdroj.dok. Biophysical Journal. - : Cell Press - ISSN 0006-3495
Roč. 122, č. 11 (2023), s. 2203-2215Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova single-particle tracking ; plasma membrane ; lateral diffusion Obor OECD Physical chemistry Způsob publikování Omezený přístup Institucionální podpora UOCHB-X - RVO:61388963 UT WOS 001019679000001 EID SCOPUS 85149707316 DOI 10.1016/j.bpj.2023.01.001 Anotace The precise spatiotemporal control of nanoscale membrane shape and composition is the result of a complex interplay of individual and collective molecular behaviors. Here, we employed single-molecule localization microscopy and computational simulations to observe single-lipid diffusion and sorting in model membranes with varying compositions, phases, temperatures, and curvatures. Supported lipid bilayers were created over 50-nm-radius nanoparticles to mimic the size of naturally occurring membrane buds, such as endocytic pits and the formation of viral envelopes. The curved membranes recruited liquid-disordered lipid phases while altering the diffusion and sorting of tracer lipids. Disorder-preferring fluorescent lipids sorted to and experienced faster diffusion on the nanoscale curvature only when embedded in a membrane capable of sustaining lipid phase separation at low temperatures. The curvature-induced sorting and faster diffusion even occurred when the sample temperature was above the miscibility temperature of the planar membrane, implying that the nanoscale curvature could induce phase separation in otherwise homogeneous membranes. Further confirmation and understanding of these results are provided by continuum and coarse-grained molecular dynamics simulations with explicit and spontaneous curvature-phase coupling, respectively. The curvature-induced membrane compositional heterogeneity and altered dynamics were achieved only with a coupling of the curvature with a lipid phase separation. These cross-validating results demonstrate the complex interplay of lipid phases, molecular diffusion, and nanoscale membrane curvature that are critical for membrane functionality. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Rok sběru 2024 Elektronická adresa https://doi.org/10.1016/j.bpj.2023.01.001
Počet záznamů: 1