The transmission and toxicity of polymer-bound doxorubicin-containing exosomes derived from human adenocarcinoma cells

Gunár, Kristýna; Kotrchová, Lenka; Filipová, Marcela; Krunclová, Tereza; Dydowiczová, Aneta; Pola, Robert; Randárová, Eva; Etrych, Tomáš; Janoušková, Olga

doi:https://dx.doi.org/10.2217/nnm-2022-0081

Počet záznamů: 1

The transmission and toxicity of polymer-bound doxorubicin-containing exosomes derived from human adenocarcinoma cells

1.

SYSNO ASEP	0564363
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	The transmission and toxicity of polymer-bound doxorubicin-containing exosomes derived from human adenocarcinoma cells
Tvůrce(i)	Gunár, Kristýna (UMCH-V)_{RID, ORCID} Kotrchová, Lenka (UMCH-V)_{RID, ORCID} Filipová, Marcela (UMCH-V)_{RID, ORCID} Krunclová, Tereza (UMCH-V)_ORCID Dydowiczová, Aneta (UMCH-V)_ORCID Pola, Robert (UMCH-V)_{RID, ORCID} Randárová, Eva (UMCH-V)_RID Etrych, Tomáš (UMCH-V)_{RID, ORCID} Janoušková, Olga (UMCH-V)_{RID, SAI, ORCID}
Zdroj.dok.	Nanomedicine. - : Future Medicine - ISSN 1743-5889 Roč. 17, č. 19 (2022), s. 1307-1322
Poč.str.	16 s.
Jazyk dok.	eng - angličtina
Země vyd.	GB - Velká Británie
Klíč. slova	cytotoxicity ; drug delivery ; extracellular vesicles
Vědní obor RIV	CD - Makromolekulární chemie
Obor OECD	Polymer science
CEP	LTAUSA18083 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Způsob publikování	Omezený přístup
Institucionální podpora	UMCH-V - RVO:61389013
UT WOS	000869390900001
EID SCOPUS	85142403255
DOI	10.2217/nnm-2022-0081
Anotace	Exosomes are extracellular vesicles with the ability to encapsulate bioactive molecules, such as therapeutics. This study identified a new exosome mediated route of doxorubicin and poly(N-(2-hydroxypropyl)methacrylamide) (pHPMA)-bound doxorubicin trafficking in the tumor mass. Exosome loading was achieved via incubation of the therapeutics with an adherent human breast adenocarcinoma cell line and its derived spheroids. Exosomes were characterized using HPLC, nanoparticle tracking analysis (NTA) and western blotting. The therapeutics were successfully loaded into exosomes. Spheroids secreted significantly more exosomes than adherent cells and showed decreased viability after treatment with therapeutic-loaded exosomes, which confirmed successful transmission. To the best of our knowledge, this study provides the first evidence of pHPMA-drug conjugate secretion by extracellular vesicles.
Pracoviště	Ústav makromolekulární chemie
Kontakt	Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358
Rok sběru	2023
Elektronická adresa	https://www.futuremedicine.com/doi/10.2217/nnm-2022-0081

Počet záznamů: 1