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TGF beta-induced changes in membrane curvature influence Ras oncoprotein membrane localization
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SYSNO ASEP 0560339 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název TGF beta-induced changes in membrane curvature influence Ras oncoprotein membrane localization Tvůrce(i) Damalas, A. (DK)
Vonková, Ivana (UMG-J)
Tutkus, M. (DK)
Stamou, D. (DK)Celkový počet autorů 4 Číslo článku 13486 Zdroj.dok. Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 12, č. 1 (2022)Poč.str. 11 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova h-ras ; plasma-membrane ; transforming growth factor beta 1 ; meselchymal transition ; proteins ; progression ; association ; activation ; carcinoma Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Cell biology Způsob publikování Open access Institucionální podpora UMG-J - RVO:68378050 UT WOS 000836707300054 DOI 10.1038/s41598-022-17482-8 Anotace In the course of cancer progression tumor cells undergo morphological changes that lead to increased motility and invasiveness thus promoting formation of metastases. This process called epithelial to mesenchymal transition (EMT) is triggered by transforming growth factor (TGF beta) but for gaining the full invasive potential an interplay between signaling of TGF beta and Ras GTPases is required. Ras proteins possess a lipidated domain that mediates Ras association with the plasma membrane, which is essential for Ras biological functions. Type and number of the lipid anchors are the main difference among three Ras variants-H-ras, N-ras and K-ras. The lipid anchors determine membrane partitioning of lipidated proteins into membrane areas of specific physico-chemical properties and curvature. In this study, we investigated the effect of TGF beta treatment on the subcellular localization of H-ras and K-ras. We show that TGF beta increases positive plasma membrane curvature, which is subsequently sensed by H-ras, leading to its elevated plasma membrane localization and activation. This observation suggests the existence of a novel positive feedback loop whereby the increased level of plasma membrane curvature during TGF beta induced EMT attracts more Ras molecules to the plasma membrane resulting in increased Ras activity which in turn promotes further EMT and thus ultimately enables the acquisition of full invasive potential. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2023 Elektronická adresa https://www.nature.com/articles/s41598-022-17482-8
Počet záznamů: 1