Počet záznamů: 1
Nicotinic Acetylcholine Receptors Expressed by Striatal Interneurons Inhibit Striatal Activity and Control Striatal-Dependent Behaviors
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SYSNO ASEP 0559170 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Nicotinic Acetylcholine Receptors Expressed by Striatal Interneurons Inhibit Striatal Activity and Control Striatal-Dependent Behaviors Tvůrce(i) Abbondanza, A. (CZ)
Bas, I.R. (CZ)
Modrák, M. (CZ)
Čapek, Martin (UMG-J)
Minich, J. (CZ)
Tyshkevich, A. (CZ)
Naser, S. (CZ)
Rangotis, R. (CZ)
Houdek, P. (CZ)
Sumova, A. (CZ)
Dumas, S. (FR)
Bernard, V. (FR)
Janíčková, H. (CZ)Celkový počet autorů 13 Zdroj.dok. Journal of Neuroscience. - : Society for Neuroscience - ISSN 0270-6474
Roč. 42, č. 13 (2022), s. 2786-2803Poč.str. 18 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova acetylcholine ; behavior ; Fos ; interneurons ; nAChRs ; striatum Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Neurosciences (including psychophysiology CEP LM2018131 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2015062 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2018129 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EF18_046/0016045 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Výzkumná infrastruktura ELIXIR-CZ II - 90131 - Ústav organické chemie a biochemie AV ČR, v. v. i. Způsob publikování Open access Institucionální podpora UMG-J - RVO:68378050 UT WOS 000779618200003 DOI 10.1523/JNEUROSCI.1627-21.2022 Anotace Acetylcholine is an important modulator of striatal activity, and it is vital to controlling striatal-dependent behaviors, including motor and cognitive functions. Despite this significance, the mechanisms determining how acetylcholine impacts striatal signaling are still not fully understood. In particular, little is known about the role of nAChRs expressed by striatal interneurons. In the present study, we used FISH to determine which neuronal types express the most prevalent beta2 nicotinic subunit in the mouse striatum. Our data support a common view that nAChR expression is mostly restricted to striatal interneurons. Surprisingly though, cholinergic interneurons were identified as a population with the highest expression of beta2 nicotinic subunit. To investigate the functional significance of beta2containing nAChRs in striatal interneurons, we deleted them by injecting the AAV-Cre vector into the striatum of beta2-flox/flox male mice. The deletion led to alterations in several behavioral domains, namely, to an increased anxiety-like behavior, decrease in sociability ratio, deficit in discrimination learning, and increased amphetamine-induced hyperlocomotion and c-Fos expression in mice with beta2 deletion. Further colocalization analysis showed that the increased c-Fos expression was present in both medium spiny neurons and presumed striatal interneurons. The present study concludes that, despite being relatively rare, beta2-containing nAChRs are primarily expressed in striatal neurons by cholinergic interneurons and play a significant role in behavior. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2023 Elektronická adresa https://www.jneurosci.org/content/42/13/2786.long
Počet záznamů: 1