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The mycobacterial guaB1 gene encodes a guanosine 5 '-monophosphate reductase with a cystathionine-beta-synthase domain
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SYSNO ASEP 0556977 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The mycobacterial guaB1 gene encodes a guanosine 5 '-monophosphate reductase with a cystathionine-beta-synthase domain Tvůrce(i) Knejzlík, Zdeněk (UOCHB-X)
Doležal, Michal (UOCHB-X) RID
Herkommerová, Klára (UOCHB-X)
Clarová, Kamila (UOCHB-X) ORCID
Klíma, Martin (UOCHB-X) RID, ORCID
Dedola, Matteo (UOCHB-X)
Zborníková, Eva (UOCHB-X) RID, ORCID
Rejman, Dominik (UOCHB-X) RID, ORCID
Pichová, Iva (UOCHB-X) RID, ORCIDZdroj.dok. FEBS Journal - ISSN 1742-464X
Roč. 289, č. 18 (2022), s. 5571-5598Poč.str. 28 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova CBS domain ; GMPR ; guaB1 ; Mycobacterium ; purine biosynthesis Obor OECD Biochemistry and molecular biology CEP EF16_019/0000729 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora UOCHB-X - RVO:61388963 UT WOS 000778625500001 EID SCOPUS 85127597203 DOI 10.1111/febs.16448 Anotace Mycobacteria express enzymes from both the de novo and purine-salvage pathways. However, the regulation of these processes and the roles of individual metabolic enzymes have not been sufficiently detailed. Both Mycobacterium tuberculosis (Mtb) and Mycobacterium smegmatis (Msm) possess three guaB genes, but information is only available on guaB2, which encodes an essential inosine 5'-monophosphate dehydrogenase (IMPDH) involved in de novo purine biosynthesis. This study shows that guaB1, annotated in databases as a putative IMPDH, encodes a guanosine 5'-monophosphate reductase (GMPR), which recycles guanosine monophosphate to inosine monophosphate within the purine-salvage pathway and contains a cystathionine-beta-synthase domain (CBS), which is essential for enzyme activity. GMPR activity is allosterically regulated by the ATP/GTP ratio in a pH-dependent manner. Bioinformatic analysis has indicated the presence of GMPRs containing CBS domains across the entire Actinobacteria phylum. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Rok sběru 2023 Elektronická adresa https://doi.org/10.1111/febs.16448
Počet záznamů: 1