Počet záznamů: 1
Nanomechanical mechanisms of Lyme disease spirochete motility enhancement in extracellular matrix
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SYSNO ASEP 0555033 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Nanomechanical mechanisms of Lyme disease spirochete motility enhancement in extracellular matrix Tvůrce(i) Strnad, Martin (BC-A) ORCID
Oh, Y. (AT)
Vancová, Marie (BC-A) RID, ORCID
Hain, L. (AT)
Salo, J. (FI)
Grubhoffer, Libor (BC-A) RID, ORCID
Nebesářová, Jana (BC-A) RID, ORCID
Hytönen, J. (FI)
Hinterdorfer, P. (AT)
Rego, Ryan O. M. (BC-A) RID, ORCIDCelkový počet autorů 10 Číslo článku 268 Zdroj.dok. Communications Biology. - : Nature Publishing Group
Roč. 4, č. 1 (2021)Poč.str. 9 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Biomedicine ; Lyme disease ; spirochete Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology CEP 8J19AT009 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy GA17-21244S GA ČR - Grantová agentura ČR LM2015062 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy EF16_013/0001775 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Způsob publikování Open access Institucionální podpora BC-A - RVO:60077344 UT WOS 000626076500001 EID SCOPUS 85102223579 DOI 10.1038/s42003-021-01783-1 Anotace As opposed to pathogens passively circulating in the body fluids of their host, pathogenic species within the Spirochetes phylum are able to actively coordinate their movement in the host to cause systemic infections. Based on the unique morphology and high motility of spirochetes, we hypothesized that their surface adhesive molecules might be suitably adapted to aid in their dissemination strategies. Designing a system that mimics natural environmental signals, which many spirochetes face during their infectious cycle, we observed that a subset of their surface proteins, particularly Decorin binding protein (Dbp) A/B, can strongly enhance the motility of spirochetes in the extracellular matrix of the host. Using single-molecule force spectroscopy, we disentangled the mechanistic details of DbpA/B and decorin/laminin interactions. Our results show that spirochetes are able to leverage a wide variety of adhesion strategies through force-tuning transient molecular binding to extracellular matrix components, which concertedly enhance spirochetal dissemination through the host. Martin Strnad, Yoo Jin Oh, and colleagues use single-molecule force spectroscopy and an extracellular matrix (ECM) analog that mimics natural tick feeding to show that the surface proteins DbpA/B can enhance spirochete motility in the ECM of the host. These results show that spirochetes can tune their transient molecular binding to ECM components to enhance spirochetal dissemination through the host. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2022 Elektronická adresa https://www.nature.com/articles/s42003-021-01783-1
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