Počet záznamů: 1
Parasitic Intestinal Protists of Zoonotic Relevance Detected in Pigs by Metabarcoding and Real-Time PCR
- 1.
SYSNO ASEP 0555031 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Parasitic Intestinal Protists of Zoonotic Relevance Detected in Pigs by Metabarcoding and Real-Time PCR Tvůrce(i) Stensvold, C.R. (DK)
Jirků-Pomajbíková, Kateřina (BC-A) ORCID, RID
Tams, K. (DK)
Jokelainen, P. (DK)
Berg, R. P. K. D. (DK)
Marving, E. (DK)
Petersen, R. (DK)
Andersen, L.O. (DK)
Angen, Ø. (DK)
Nielsen, H.V. (DK)Celkový počet autorů 10 Číslo článku 1189 Zdroj.dok. Microorganisms. - : MDPI
Roč. 9, č. 6 (2021)Poč.str. 16 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova genetic diversity ; dientamoeba-fragilis ; enterocytozoon-bieneusi ; lawsonia-intracellularis ; nursery pigs ; blastocystis ; prevalence ; cryptosporidium ; balantidium ; population ; metabarcoding ; next-generation sequencing ; zoonotic infections ; parasite ; parasitology ; host specificity ; genetic diversity ; dna ; pcr Vědní obor RIV EE - Mikrobiologie, virologie Obor OECD Microbiology Způsob publikování Open access Institucionální podpora BC-A - RVO:60077344 UT WOS 000666160700001 EID SCOPUS 85106873937 DOI 10.3390/microorganisms9061189 Anotace Several parasite species are shared between humans and pigs. We explored the application of next-generation sequencing-based metabarcoding supplemented with real-time PCR to fecal DNAs from 259 samples from 116 pigs in Denmark to detect and differentiate single-celled intestinal parasites of zoonotic relevance. Enterocytozoon bieneusi, Balantioides coli, and Giardia duodenalis were observed in 34/37 (92%), 148/259 (57%), and 86/259 (33%) samples, respectively. Entamoeba polecki ST1, E. polecki ST3, and Entamoeba hartmanni were detected in 104/259 (40%), 161/259 (62%), and 8/259 (3%) samples, respectively. Metabarcoding and real-time PCR detected Cryptosporidium in 90/259 (35%) and 239/259 (92%) of the samples, respectively, with Cryptosporidium suis and Cryptosporidium scrofarum observed in nearly equal proportions. Blastocystis subtypes 1, 3, 5, and 15 were found in 72 (28%), 6 (2%), 176 (68%), and 36 (14%) of 259 samples, respectively. Iodamoeba was identified in 1/259 samples (<1%), while none of 37 tested samples was positive for Dientamoeba fragilis. Our results illustrate how metabarcoding exemplifies a 'one-fits-many' approach to detecting intestinal single-celled parasites in feces supplemented with real-time PCR for selected parasites. Using metabarcoding with pathogen-specific assays may help detect emerging and previously underdetected pathogens and further elucidate the role of micro-eukaryotic parasites in human and animal health and disease. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2022 Elektronická adresa https://www.mdpi.com/2076-2607/9/6/1189
Počet záznamů: 1