Počet záznamů: 1
Early perturbation of Wnt signaling reveals patterning and invagination-evagination control points in molar tooth development
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SYSNO ASEP 0554914 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Early perturbation of Wnt signaling reveals patterning and invagination-evagination control points in molar tooth development Tvůrce(i) Kim, R. (US)
Yu t, T. (US)
Li, J. (GB)
Procházka, Jan (UMG-J) ORCID
Sharir, A. (US)
Green j, J.B.A. (US)
Klein, O. (US)Celkový počet autorů 7 Číslo článku dev199685 Zdroj.dok. Development. - : Company of Biologists - ISSN 0950-1991
Roč. 148, č. 14 (2021)Poč.str. 10 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova Tooth development ; Epithelial invagination ; Morphogenesis ; Wnt signaling ; Mouse Vědní obor RIV EB - Genetika a molekulární biologie Obor OECD Developmental biology Způsob publikování Omezený přístup Institucionální podpora UMG-J - RVO:68378050 UT WOS 000681398800018 DOI 10.1242/dev.199685 Anotace Tooth formation requires complex signaling interactions both within the oral epithelium and between the epithelium and the underlying mesenchyme. Previous studies of the Wnt/beta-catenin pathway have shown that tooth formation is partly inhibited in loss-of-function mutants, and gain-of-function mutants have perturbed tooth morphology. However, the stage at which Wnt signaling is first important in tooth formation remains unclear. Here, using an Fgf8-promoter-driven, and therefore early, deletion of beta-catenin in mouse molar epithelium, we found that loss of Wnt/beta-catenin signaling completely deletes the molar tooth, demonstrating that this pathway is central to the earliest stages of tooth formation. Early expression of a dominant-active p-catenin protein also perturbs tooth formation, producing a large domed evagination at early stages and supernumerary teeth later on. The early evaginations are associated with premature mesenchymal condensation marker, and are reduced by inhibition of condensation-associated collagen synthesis. We propose that invagination versus evagination morphogenesis is regulated by the relative timing of epithelial versus mesenchymal cell convergence regulated by canonical Wnt signaling. Together, these studies reveal new aspects of Wnt/beta-catenin signaling in tooth formation and in epithelial morphogenesis more broadly. Pracoviště Ústav molekulární genetiky Kontakt Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Rok sběru 2022 Elektronická adresa https://journals.biologists.com/dev/article/148/14/dev199685/271019/Early-perturbation-of-Wnt-signaling-reveals
Počet záznamů: 1