Počet záznamů: 1
Detection of Anaplasma phagocytophilum in European brown hares (Lepus europaeus) using three different methods
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SYSNO ASEP 0554280 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Detection of Anaplasma phagocytophilum in European brown hares (Lepus europaeus) using three different methods Tvůrce(i) Lesiczka, P. (CZ)
Modrý, David (BC-A) RID, ORCID
Sprong, H. (NL)
Fonville, M. (NL)
Pikula, J. (CZ)
Piaček, V. (CZ)
Heger, T. (CZ)
Hrazdilová, K. (CZ)Celkový počet autorů 8 Zdroj.dok. Zoonoses and Public Health. - : Wiley - ISSN 1863-1959
Roč. 68, č. 8 (2021), s. 917-925Poč.str. 9 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova pcr ; animals ; Anaplasma phagocytophilum ; digital droplet PCR ; European brown hare ; Lepus europaeus ; qPCR ; zoonosis Vědní obor RIV EG - Zoologie Obor OECD Zoology CEP QK1920258 GA MZe - Ministerstvo zemědělství Způsob publikování Omezený přístup Institucionální podpora BC-A - RVO:60077344 UT WOS 000683888600001 EID SCOPUS 85112245550 DOI 10.1111/zph.12883 Anotace European brown hare (Lepus europaeus Pallas 1778) is a broadly distributed lagomorph species in Europe, recognized as a host for Ixodes ricinus and reservoir of a wide range of pathogens with zoonotic potential. Even though Lepus europaeus represents an important game animal in Central Europe, the data available on Anaplasma phagocytophilum in this lagomorph are scarce. In this study, three populations of brown hare from distinct localities in the Czech Republic were analysed for the presence of Anaplasma phagocytophilum DNA. We used standard qPCR, targeting the msp2 gene and adapted the same assay also for digital droplet PCR. Out of 91 samples, these two methods identified 9 and 12 as positive, respectively. For taxonomic analysis, we amplified the groEL gene from five of six samples that were found positive by both methods. In phylogenetic analyses, this haplotype belongs to ecotype 1, and to the subclade with isolates from cervids and I. ricinus. Our findings underline the importance of correct result interpretation and positivity cut-off set-up for different detection methods of A. phagocytophilum. This bacterium is characterized by a high intraspecific variability and highly sensitive detection itself, is not enough. Detailed molecular typing is necessary to define the zoonotic potential of different strains and their natural reservoirs. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2022 Elektronická adresa https://onlinelibrary.wiley.com/doi/10.1111/zph.12883
Počet záznamů: 1