Ordinary Gasoline Emissions Induce a Toxic Response in Bronchial Cells Grown at Air-Liquid Interface

Červená, Tereza; Vojtíšek-Lom, M.; Vrbová, Kristýna; Ambrož, Antonín; Nováková, Zuzana; Elzeinová, Fatima; Šíma, Michal; Beránek, V.; Pechout, M.; Macoun, D.; Kléma, J.; Rössnerová, Andrea; Cigánek, M.; Topinka, Jan; Rössner ml., Pavel

doi:https://dx.doi.org/10.3390/ijms22010079

Počet záznamů: 1

Ordinary Gasoline Emissions Induce a Toxic Response in Bronchial Cells Grown at Air-Liquid Interface

1.

SYSNO ASEP	0551562
Druh ASEP	J - Článek v odborném periodiku
Zařazení RIV	J - Článek v odborném periodiku
Poddruh J	Článek ve WOS
Název	Ordinary Gasoline Emissions Induce a Toxic Response in Bronchial Cells Grown at Air-Liquid Interface
Tvůrce(i)	Červená, Tereza (UEM-P)_{ORCID, RID} Vojtíšek-Lom, M. (CZ) Vrbová, Kristýna (UEM-P) Ambrož, Antonín (UEM-P) Nováková, Zuzana (UEM-P) Elzeinová, Fatima (UEM-P) Šíma, Michal (UEM-P)_{RID, ORCID} Beránek, V. (CZ) Pechout, M. (CZ) Macoun, D. (CZ) Kléma, J. (CZ) Rössnerová, Andrea (UEM-P)_RID Cigánek, M. (CZ) Topinka, Jan (UEM-P)_{RID, ORCID} Rössner ml., Pavel (UEM-P)_{RID, ORCID}
Číslo článku	79
Zdroj.dok.	International Journal of Molecular Sciences. - : MDPI Roč. 22, č. 1 (2021)
Poč.str.	22 s.
Jazyk dok.	eng - angličtina
Země vyd.	CH - Švýcarsko
Klíč. slova	gasoline emissions ; toxicity ; air-liquid interface ; MucilAir (TM) ; bronchial epithelial cells
Vědní obor RIV	EI - Biotechnologie a bionika
Obor OECD	Toxicology
CEP	GA18-04719S GA ČR - Grantová agentura ČR
	LM2018124 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
	EF18_046/0015586 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy
Způsob publikování	Open access
Institucionální podpora	UEM-P - RVO:68378041
UT WOS	000606114000001
EID SCOPUS	85098623220
DOI	10.3390/ijms22010079
Anotace	Gasoline engine emissions have been classified as possibly carcinogenic to humans and represent a significant health risk. In this study, we used MucilAir (TM), a three-dimensional (3D) model of the human airway, and BEAS-2B, cells originating from the human bronchial epithelium, grown at the air-liquid interface to assess the toxicity of ordinary gasoline exhaust produced by a direct injection spark ignition engine. The transepithelial electrical resistance (TEER), production of mucin, and lactate dehydrogenase (LDH) and adenylate kinase (AK) activities were analyzed after one day and five days of exposure. The induction of double-stranded DNA breaks was measured by the detection of histone H2AX phosphorylation. Next-generation sequencing was used to analyze the modulation of expression of the relevant 370 genes. The exposure to gasoline emissions affected the integrity, as well as LDH and AK leakage in the 3D model, particularly after longer exposure periods. Mucin production was mostly decreased with the exception of longer BEAS-2B treatment, for which a significant increase was detected. DNA damage was detected after five days of exposure in the 3D model, but not in BEAS-2B cells. The expression of CYP1A1 and GSTA3 was modulated in MucilAir (TM) tissues after 5 days of treatment. In BEAS-2B cells, the expression of 39 mRNAs was affected after short exposure, most of them were upregulated. The five days of exposure modulated the expression of 11 genes in this cell line. In conclusion, the ordinary gasoline emissions induced a toxic response in MucilAir (TM). In BEAS-2B cells, the biological response was less pronounced, mostly limited to gene expression changes.
Pracoviště	Ústav experimentální medicíny
Kontakt	Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218
Rok sběru	2022
Elektronická adresa	https://www.mdpi.com/1422-0067/22/1/79

Počet záznamů: 1