Počet záznamů: 1
The Distinct Function and Localization of METTL3/METTL14 and METTL16 Enzymes in Cardiomyocytes
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SYSNO ASEP 0537440 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název The Distinct Function and Localization of METTL3/METTL14 and METTL16 Enzymes in Cardiomyocytes Tvůrce(i) Arcidiacono, Orazio Angelo (BFU-R) ORCID
Krejčí, Jana (BFU-R) RID, ORCID
Bártová, Eva (BFU-R) ORCIDCelkový počet autorů 3 Číslo článku 8139 Zdroj.dok. International Journal of Molecular Sciences. - : MDPI
Roč. 21, č. 21 (2020)Poč.str. 19 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. CH - Švýcarsko Klíč. slova messenger-rna methylation ; embryonic stem-cells ; cardiac-hypertrophy ; gene-expression ; m(6)a methyltransferase ; histone deacetylase-1 ; hdac inhibitors ; tet proteins Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology CEP GA18-07384S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora BFU-R - RVO:68081707 UT WOS 000588866000001 EID SCOPUS 85095575320 DOI 10.3390/ijms21218139 Anotace It has become evident that epitranscriptome events, mediated by specific enzymes, regulate gene expression and, subsequently, cell differentiation processes. We show that methyltransferase-like proteins METTL3/METTL14 and N-6-adenosine methylation (m6A) in RNAs are homogeneously distributed in embryonic hearts, and histone deacetylase (HDAC) inhibitors valproic acid and Trichostatin A (TSA) up-regulate METTL3/METTL14 proteins. The levels of METTL3 in mouse adult hearts, isolated from male and female animals, were lower in the aorta and pulmonary trunks when compared with atria, but METT14 was up-regulated in the aorta and pulmonary trunk, in comparison with ventriculi. Aging caused METTL3 down-regulation in aorta and atria in male animals. Western blot analysis in differentiated mouse embryonic stem cells (mESCs), containing 10-30 percent of cardiomyocytes, showed METTL3/METTL14 down-regulation, while the differentiation-induced increased level of METTL16 was observed in both wild type (wt) and HDAC1 depleted (dn) cells. In parallel, experimental differentiation in especially HDAC1 wild type cells was accompanied by depletion of m6A in RNA. Immunofluorescence analysis of individual cells revealed the highest density of METTL3/METTL14 in alpha-actinin positive cardiomyocytes when compared with the other cells in the culture undergoing differentiation. In both wt and HDAC1 dn cells, the amount of METTL16 was also up-regulated in cardiomyocytes when compared to co-cultivated cells. Together, we showed that distinct anatomical regions of the mouse adult hearts are characterized by different levels of METTL3 and METTL14 proteins, which are changed during aging. Experimental cell differentiation was also accompanied by changes in METTL-like proteins and m6A in RNA, in particular, levels and distribution patterns of METTL3/METTL14 proteins were different from the same parameters studied in the case of the METTL16 protein. Pracoviště Biofyzikální ústav Kontakt Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Rok sběru 2021 Elektronická adresa http://apps.webofknowledge.com/InboundService.do?customersID=Alerting&mode=FullRecord&IsProductCode=Yes&product=WOS&Init=Yes&Func=Frame&DestFail=http%3A%2F%2Fwww.webofknowledge.com&action=retrieve&SrcApp=Alerting&SrcAuth=Alerting&SID=F5ecl7TTnxXPl2FJDDH&UT=WOS%3A000588866000001
Počet záznamů: 1