Počet záznamů: 1
Reconstruction of the absorption spectrum of Synechocystis sp. PCC 6803 optical mutants from the in vivo signature of individual pigments
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SYSNO ASEP 0537134 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Reconstruction of the absorption spectrum of Synechocystis sp. PCC 6803 optical mutants from the in vivo signature of individual pigments Tvůrce(i) Fuente Herraiz, David (UEK-B)
Lazár, D. (CZ)
Oliver-Villanueva, J. V. (ES)
Urchueguía, J. F. (ES)Celkový počet autorů 4 Zdroj.dok. Photosynthesis Research. - : Springer - ISSN 0166-8595
Roč. 147, č. 1 (2021), s. 75-90Poč.str. 15 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova photosystem-i ; wavelength dependence ; state transitions ; light-absorption ; chlorophyll-a ; sp pcc-6803 ; carotenoids ; phytoplankton ; growth ; myxoxanthophyll ; Absorption ; Spectrum ; Light ; Pigment ; Modeling ; Synechocystis ; Photosystem Vědní obor RIV BO - Biofyzika Obor OECD Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology Výzkumná infrastruktura CzeCOS III - 90123 - Ústav výzkumu globální změny AV ČR, v. v. i. Způsob publikování Omezený přístup Institucionální podpora UEK-B - RVO:86652079 UT WOS 000593428700001 EID SCOPUS 85096880710 DOI 10.1007/s11120-020-00799-8 Anotace In this work, we reconstructed the absorption spectrum of different Synechocystis sp. PCC 6803 optical strains by summing the computed signature of all pigments present in this organism. To do so, modifications to in vitro pigment spectra were first required: namely wavelength shift, curve smoothing, and the package effect calculation derived from high pigment densities were applied. As a result, we outlined a plausible shape for the in vivo absorption spectrum of each chromophore. These are flatter and slightly broader in physiological conditions yet the mean weight-specific absorption coefficient remains identical to the in vitro conditions. Moreover, we give an estimate of all pigment concentrations without applying spectrophotometric correlations, which are often prone to error. The computed cell spectrum reproduces in an accurate manner the experimental spectrum for all the studied wavelengths in the wild-type, Olive, and PAL strain. The gathered pigment concentrations are in agreement with reported values in literature. Moreover, different illumination set-ups were evaluated to calculate the mean absorption cross-section of each chromophore. Finally, a qualitative estimate of light-limited cellular growth at each wavelength is given. This investigation describes a novel way to approach the cell absorption spectrum and shows all its inherent potential for photosynthesis research. Pracoviště Ústav výzkumu globální změny Kontakt Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Rok sběru 2022 Elektronická adresa https://link.springer.com/article/10.1007/s11120-020-00799-8
Počet záznamů: 1