Homozygous transgenic barley (Hordeum vulgare L.) plants by anther culture

0532719 - ÚEB 2021 RIV CH eng J - Článek v odborném periodiku
Ohnoutková, Ludmila - Vlčko, Tomáš
Homozygous transgenic barley (Hordeum vulgare L.) plants by anther culture.
Plants. Roč. 9, č. 7 (2020), s. 1-11, č. článku 918. E-ISSN 2223-7747
Grant CEP: GA MŠMT(CZ) EF16_019/0000738
Institucionální podpora: RVO:61389030
Klíčová slova: Androgenesis barley * Double haploid * Embryo culture * Transformation
Obor OECD: Biochemical research methods
Impakt faktor: 3.935, rok: 2020
Způsob publikování: Open access
http://doi.org/10.3390/plants9070918

Production of homozygous lines derived from transgenic plants is one of the important steps for phenotyping and genotyping transgenic progeny. The selection of homozygous plants is a tedious process that can be significantly shortened by androgenesis, cultivation of anthers, or isolated microspores. Doubled haploid (DH) production achieves complete homozygosity in one generation. We obtained transgenic homozygous DH lines from six different transgenic events by using anther culture. Anthers were isolated from T0 transgenic primary regenerants and cultivated in vitro. The ploidy level was determined in green regenerants. At least half of the 2n green plants were transgenic, and their progeny were shown to carry the transgene. The process of dihaploidization did not affect the expression of the transgene. Embryo cultures were used to reduce the time to seed of the next generation. The application of these methods enables rapid evaluation of transgenic lines for gene function studies and trait evaluation.
Trvalý link: http://hdl.handle.net/11104/0311131