Počet záznamů: 1
Differential pulse voltammetric determination of homovanillic acid as a tumor biomarker in human urine after hollow fiber-based liquid-phase microextraction
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SYSNO ASEP 0532424 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Differential pulse voltammetric determination of homovanillic acid as a tumor biomarker in human urine after hollow fiber-based liquid-phase microextraction Tvůrce(i) Hrdlička, Vojtěch (UFCH-W) ORCID, RID, SAI
Barek, J. (CZ)
Navrátil, Tomáš (UFCH-W) RID, ORCIDČíslo článku 121594 Zdroj.dok. Talanta. - : Elsevier - ISSN 0039-9140
Roč. 221, JAN 2021 (2021)Poč.str. 7 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova Liquid phase microextraction ; Homovanillic acid ; Hollow fiber ; Differential pulse voltammetry Vědní obor RIV CG - Elektrochemie Obor OECD Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis) CEP GA20-01589S GA ČR - Grantová agentura ČR Způsob publikování Omezený přístup Institucionální podpora UFCH-W - RVO:61388955 UT WOS 000579910600084 EID SCOPUS 85091253335 DOI 10.1016/j.talanta.2020.121594 Anotace Novel method for the determination of a tumor marker homovanillic acid (HVA) in human urine was developed. Combination of hollow fiber – based liquid-phase microextraction (HF-LPME) and differential pulse voltammetry (DPV) at a cathodically pre-treated boron doped diamond electrode (BDDE) was applied for these purposes. Optimum conditions were: butyl benzoate as supported liquid membrane (SLM) formed on polypropylene HF, 0.1 mol L−1 HCl as donor phase, 0.1 mol L−1 sodium phosphate buffer of pH 6 as acceptor phase, and 30 min extraction time. HF-LPME-DPV concentration dependence was linear in the range from 1.2 to 100 μmol L−1. Limits of quantification (LOQ) and detection (LOD) were 1.2 and 0.4 μmol L−1, respectively. The applicability of the developed method was verified by analysis of human urine. Standard addition method was used, found HVA concentration was 13.5 ± 1.3 μmol L−1, RSD = 9.3% (n=5). Pracoviště Ústav fyzikální chemie J.Heyrovského Kontakt Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Rok sběru 2022 Elektronická adresa http://hdl.handle.net/11104/0310920
Počet záznamů: 1