Počet záznamů: 1
Transcriptional profiling of human macrophages during infection with Bordetella pertussis
- 1.0524572 - MBÚ 2021 RIV US eng J - Článek v odborném periodiku
Petráčková, Denisa - Farman, M.R. - Amman, F. - Linhartová, Irena - Dienstbier, Ana - Kumar, Dilip - Držmíšek, Jakub - Hofacker, I. - Rodriguez, E.M. - Večerek, Branislav
Transcriptional profiling of human macrophages during infection with Bordetella pertussis.
RNA biology. Roč. 17, č. 5 (2020), s. 731-742. ISSN 1547-6286. E-ISSN 1555-8584
Grant CEP: GA ČR(CZ) GF16-34825L; GA ČR(CZ) GA19-12338S; GA MZd(CZ) NV16-30782A; GA MŠMT(CZ) EF16_027/0007990
Institucionální podpora: RVO:61388971
Klíčová slova: Bordetella pertussis * infection * macrophage
Obor OECD: Microbiology
Impakt faktor: 4.652, rok: 2020
Způsob publikování: Open access
https://www.tandfonline.com/doi/full/10.1080/15476286.2020.1727694
Bordetella pertussis, a strictly human re-emerging pathogen and the causative agent of whooping cough, exploits a broad variety of virulence factors to establish efficient infection. Here, we used RNA sequencing to analyse the changes in gene expression profiles of human THP-1 macrophages resulting from B. pertussis infection. In parallel, we attempted to determine the changes in intracellular B. pertussis-specific transcriptomic profiles resulting from interaction with macrophages. Our analysis revealed that global gene expression profiles in THP-1 macrophages are extensively rewired 6 h post-infection. Among the highly expressed genes, we identified those encoding cytokines, chemokines, and transcription regulators involved in the induction of the M1 and M2 macrophage polarization programmes. Notably, several host genes involved in the control of apoptosis and inflammation which are known to be hijacked by intracellular bacterial pathogens were overexpressed upon infection. Furthermore, in silico analyses identified large temporal changes in expression of specific gene subsets involved in signalling and metabolic pathways. Despite limited numbers of the bacterial reads, we observed reduced expression of majority of virulence factors and upregulation of several transcriptional regulators during infection suggesting that intracellular B. pertussis cells switch from virulent to avirulent phase and actively adapt to intracellular environment, respectively.
Trvalý link: http://hdl.handle.net/11104/0308927
Počet záznamů: 1