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Taste for protein: Chemical signal from prey stimulates enzyme secretion through jasmonate signalling in the carnivorous plant Venus flytrap
- 1.0522617 - ÚEB 2020 RIV FR eng J - Článek v odborném periodiku
Jakšová, J. - Libiaková, M. - Bokor, B. - Petřík, Ivan - Novák, Ondřej - Pavlovič, A.
Taste for protein: Chemical signal from prey stimulates enzyme secretion through jasmonate signalling in the carnivorous plant Venus flytrap.
Plant Physiology and Biochemistry. Roč. 146, January (2020), s. 90-97. ISSN 0981-9428
Grant CEP: GA MŠMT(CZ) EF16_019/0000827
Institucionální podpora: RVO:61389030
Klíčová slova: Action potential * Carnivorous plant * Chitinase * Digestive enzyme * Jasmonic acid * Protease * Venus flytrap
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 4.270, rok: 2020
Způsob publikování: Open access
https://doi.org/10.1016/j.plaphy.2019.11.013
Hunting cycle of the carnivorous plant Venus flytrap (Dionaea muscipula Ellis) is comprised of mechanism for rapid trap closure followed by slow hermetical sealing and activation of gene expression responsible for digestion of prey and nutrient uptake. In the present study, we focus on the late phase of Venus's flytrap hunting cycle when mechanical stimulation of the prey ceases and is replaced by chemical cues. We used two nitrogen-rich compounds (chitin and protein) in addition to mechanostimulation to investigate the electrical and jasmonate signalling responsible for induction of enzyme activities. Chemical stimulation by BSA protein and chitin did not induce any additional spontaneous action potentials (APs). However, chemical stimulation by protein induced the highest levels of jasmonic acid (JA) and its isoleucine conjugate (JA-Ile) as well as the expression of studied gene encoding a cysteine protease (dionain). Although chitin is probably the first chemical agent which is in direct contact with digestive glands, presence of protein in the secured trap mimics the presence of insect prey best.
Trvalý link: http://hdl.handle.net/11104/0307082
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