Počet záznamů: 1
Rapid methods for the separation of natural mixtures of beauverolides cholesterol acyltransferase inhibitors, isolated from the fungus Isaria fumosorosea
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SYSNO ASEP 0521376 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Rapid methods for the separation of natural mixtures of beauverolides cholesterol acyltransferase inhibitors, isolated from the fungus Isaria fumosorosea Tvůrce(i) Šimčíková, D. (CZ)
Tůma, P. (CZ)
Jegorov, Alexandr (BC-A)
Šimek, Petr (BC-A) RID, ORCID
Heneberg, P. (CZ)Celkový počet autorů 5 Zdroj.dok. Journal of Separation Science. - : Wiley - ISSN 1615-9306
Roč. 43, č. 5 (2020), s. 962-969Poč.str. 8 s. Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova Alzheimer’s disease ; atherosclerosis ; capillary electrophoresis Vědní obor RIV CE - Biochemie Obor OECD Biochemical research methods Způsob publikování Omezený přístup Institucionální podpora BC-A - RVO:60077344 UT WOS 000505036400001 EID SCOPUS 85078607387 DOI 10.1002/jssc.201901084 Anotace Beauverolides (beauveriolides) are abundant, biologically active cyclodepsipeptides produced by many entomopathogenic fungi, including those that are used as biopesticides. Beauverolides act as cholesterol acyltransferase inhibitors in humans, thus, their mode of action has been the subject of pharmacological and clinical research. The cost-effective analytical methods are needed for fast, routine laboratory analysis of beauverolides. We isolated beauverolides from the fungal strain Isaria fumosorosea PFR 97-Apopka and opened the rings of the isolated beauverolides using a pyridine alkaline medium. We separated fractions of cyclic and linearized beauverolides by thin-layer chromatography, and found the chloroform-acetate (9:1, v/v) and chloroform-acetonitrile-acetate (8:1:1, v/v/v) mobile phases, respectively, to be the most efficient. We examined all the fractions by liquid chromatography-mass spectrometry using ion trap and Orbitrap high resolution mass spectrometry. For rapid screening of the contents of cyclic, and, particularly, linearized beauverolides, we developed a novel analytical method that consisted of using capillary electrophoresis coupled with contactless conductivity detection. Furthermore, we improved the separation of the peptides by applying capillary micellar electrokinetic chromatography with the N-cyclohexyl-2-aminoethanesulfonic acid:SDS:NaOH buffer, pH 9.8 as the background electrolyte. The described novel methods allow fast and cost-effective separation of chemically related groups of beauverolides. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2021 Elektronická adresa https://onlinelibrary.wiley.com/doi/full/10.1002/jssc.201901084
Počet záznamů: 1