0520853 - BC 2020 RIV US eng M - Monography Chapter
Nebesářová, Jana
Essential Methods of Plant Sample Preparation for High-Resolution Scanning Electron Microscopy at Room Temperature.
Methods in Molecular Biology. In: PLANT CELL MORPHOGENESIS: METHODS AND PROTOCOLS, 2ND EDITION. TOTOWA: HUMANA PRESS INC., 2019 - (Cvrčková, F.; Žárský, V.), s. 63-76. Methods in Molecular Biology. ISBN 978-1-4939-9469-4
R&D Projects: GA MŠMT(CZ) LM2015062; GA MŠMT(CZ) EF16_013/0001775
Institutional support: RVO:60077344
Keywords : surfaces * sem * fixation * High-resolution scanning electron microscope * Plant sample * Chemical fixation * Dehydration * Critical point drying * t-Butanol freeze drying * Metal coating * Sample storage
OECD category: Cell biology
Result website:
https://link.springer.com/protocol/10.1007/978-1-4939-9469-4_4DOI: https://doi.org/10.1007/978-1-4939-9469-4_4

In this chapter, conventional techniques are described for the preparation of plant samples at room temperature before examination in the high resolution scanning electron microscopy. Protocols are given on how to collect, to fix, to dehydrate, and to dry plant samples. Subsequently, it is described how to stick them to stubs and cover with a thin conductive layer. These methods are suitable for a wide variety of plant specimens, ranging from microalgae to higher plants.
Permanent Link: http://hdl.handle.net/11104/0305513