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Chemoenzymatic Synthesis and Radical Scavenging of Sulfated Hydroxytyrosol, Tyrosol, and Acetylated Derivatives
- 1.0507895 - MBÚ 2020 RIV US eng J - Článek v odborném periodiku
Begines, P. - Biedermann, David - Valentová, Kateřina - Petrásková, Lucie - Pelantová, Helena - Maya, I. - Fernandez-Bolanos, J.G. - Křen, Vladimír
Chemoenzymatic Synthesis and Radical Scavenging of Sulfated Hydroxytyrosol, Tyrosol, and Acetylated Derivatives.
Journal of Agricultural and Food Chemistry. Roč. 67, č. 26 (2019), s. 7281-7288. ISSN 0021-8561. E-ISSN 1520-5118
Grant CEP: GA ČR(CZ) GA18-00121S; GA ČR(CZ) GA19-00043S
Institucionální podpora: RVO:61388971
Klíčová slova: olive phenols * arylsulfotransferase * hydroxytyrosol
Obor OECD: Biochemistry and molecular biology
Impakt faktor: 4.192, rok: 2019
Způsob publikování: Omezený přístup
https://pubs.acs.org/doi/10.1021/acs.jafc.9b01065
Potential metabolites of bioactive compounds are important for their biological activities and as authentic standards for metabolic studies. The phenolic compounds contained in olive oil are an important part of the human diet, and therefore their potential metabolites are of utmost interest. We developed a convenient, scalable, one-pot chemoenzymatic method using the arylsulfotransferase from Desulfitobacterium hafniense for the sulfation of the natural olive oil phenols tyrosol, hydroxytyrosol, and of their monoacetylated derivatives. Respective monosulfated (tentative) metabolites were fully structurally characterized using LC-MS, NMR, and HRMS. In addition, Folin-Ciocalteu reduction, 1,1-diphenyl-2-picrylhydrazyl radical scavenging, and antilipoperoxidant activity in rat liver microsomes damaged by tert-butylhydroperoxide were measured and compared to the parent compounds. As expected, the sulfation diminished the radical scavenging properties of the prepared compounds. These compounds will serve as authentic standards of phase II metabolites.
Trvalý link: http://hdl.handle.net/11104/0298855
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