Počet záznamů: 1
Extreme genome diversity in the hyper-prevalent parasitic eukaryote Blastocystis
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SYSNO ASEP 0507395 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Extreme genome diversity in the hyper-prevalent parasitic eukaryote Blastocystis Tvůrce(i) Gentekaki, E. (CA)
Curtis, B.A. (CA)
Stairs, C.W. (CA)
Klimeš, V. (CZ)
Eliaš, M. (CZ)
Salas-Leiva, D.E. (CA)
Herman, E.K. (CA)
Eme, L. (CA)
Arias, M.C. (FR)
Henrissat, B. (FR)
Hilliou, F. (FR)
Klute, M.J. (CA)
Suga, H. (JP)
Malik, S.B. (CA)
Pightling, A.W. (CA)
Kolísko, Martin (BC-A) ORCID
Rachubinski, R.A. (CA)
Schlacht, A. (CA)
Soanes, D.M. (GB)
Tsaousis, A.D. (CA)
Archibald, J.M. (CA)
Ball, S.G. (FR)
Dacks, J.B. (CA)
Clark, C.G. (GB)
van der Giezen, M. (GB)
Roger, A. J. (CA)Celkový počet autorů 26 Číslo článku e2003769 Zdroj.dok. PLOS Biology. - : Public Library of Science - ISSN 1544-9173
Roč. 15, č. 9 (2017)Poč.str. 42 s. Forma vydání Online - E Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova anaphase-promoting complex ; hidden markov model ; protein-kinase ; in-vitro ; entamoeba-histolytica ; cysteine proteases ; rhomboid protease ; surface-coat ; metabolism ; sequence Vědní obor RIV EF - Botanika Obor OECD Plant sciences, botany CEP GA13-33039S GA ČR - Grantová agentura ČR Způsob publikování Open access Institucionální podpora BC-A - RVO:60077344 UT WOS 000411978200015 EID SCOPUS 85030688560 DOI 10.1371/journal.pbio.2003769 Anotace Blastocystis is the most prevalent eukaryotic microbe colonizing the human gut, infecting approximately 1 billion individuals worldwide. Although Blastocystis has been linked to intestinal disorders, its pathogenicity remains controversial because most carriers are asymptomatic. Here, the genome sequence of Blastocystis subtype (ST) 1 is presented and compared to previously published sequences for ST4 and ST7. Despite a conserved core of genes, there is unexpected diversity between these STs in terms of their genome sizes, guanine-cytosine (GC) content, intron numbers, and gene content. ST1 has 6,544 protein-coding genes, which is several hundred more than reported for ST4 and ST7. The percentage of proteins unique to each ST ranges from 6.2% to 20.5%, greatly exceeding the differences observed within parasite genera. Orthologous proteins also display extreme divergence in amino acid sequence identity between STs (i.e., 59%-61% median identity), on par with observations of the most distantly related species pairs of parasite genera. The STs also display substantial variation in gene family distributions and sizes, especially for protein kinase and protease gene families, which could reflect differences in virulence. It remains to be seen to what extent these inter-ST differences persist at the intra-ST level. A full 26% of genes in ST1 have stop codons that are created on the mRNA level by a novel polyadenylation mechanism found only in Blastocystis. Reconstructions of pathways and organellar systems revealed that ST1 has a relatively complete membrane-trafficking system and a near-complete meiotic toolkit, possibly indicating a sexual cycle. Unlike some intestinal protistan parasites, Blastocystis ST1 has near-complete de novo pyrimidine, purine, and thiamine biosynthesis pathways and is unique amongst studied stramenopiles in being able to metabolize alpha-glucans rather than beta-glucans. It lacks all genes encoding heme-containing cytochrome P450 proteins. Predictions of the mitochondrion-related organelle (MRO) proteome reveal an expanded repertoire of functions, including lipid, cofactor, and vitamin biosynthesis, as well as proteins that may be involved in regulating mitochondrial morphology and MRO/endoplasmic reticulum (ER) interactions. In sharp contrast, genes for peroxisome-associated functions are absent, suggesting Blastocystis STs lack this organelle. Overall, this study provides an important window into the biology of Blastocystis, showcasing significant differences between STs that can guide future experimental investigations into differences in their virulence and clarifying the roles of these organisms in gut health and disease. Pracoviště Biologické centrum (od r. 2006) Kontakt Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Rok sběru 2020 Elektronická adresa https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.2003769&type=printable
Počet záznamů: 1