Počet záznamů: 1
Endogenous Fatty Acids Are Essential Signaling Factors of Pancreatic beta-Cells and Insulin Secretion
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SYSNO ASEP 0495209 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Endogenous Fatty Acids Are Essential Signaling Factors of Pancreatic beta-Cells and Insulin Secretion Tvůrce(i) Hauke, S. (DE)
Keutler, K. (DE)
Phapale, P. (DE)
Yushchenko, Dmytro A. (UOCHB-X) ORCID, RID
Schultz, C. (DE)Zdroj.dok. Diabetes. - : American Diabetes Association - ISSN 0012-1797
Roč. 67, č. 10 (2018), s. 1986-1998Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova human plasma albumin ; cytoplasmic Ca2+ ; serum albumin Vědní obor RIV CE - Biochemie Obor OECD Biochemistry and molecular biology Institucionální podpora UOCHB-X - RVO:61388963 UT WOS 000445059100008 EID SCOPUS 85054776125 DOI 10.2337/db17-1215 Anotace The secretion of insulin from beta-cells depends on extracellular factors, in particular glucose and other small molecules, some of which act on G-protein-coupled receptors. Fatty acids (FAs) have been discussed as exogenous secretagogues of insulin for decades, especially after the FA receptor GPR40 (G-protein-coupled receptor 40) was discovered. However, the role of FAs as endogenous signaling factors has not been investigated until now. In the present work, we demonstrate that lowering endogenous FA levels in beta-cell medium by stringent washing or by the application of FA-free (FAF) BSA immediately reduced glucose-induced oscillations of cytosolic Ca2+ ([Ca2+](i) oscillations) in MIN6 cells and mouse primary beta-cells, as well as insulin secretion. Mass spectrometry confirmed BSA-mediated removal of FAs, with palmitic, stearic, oleic, and elaidic acid being the most abundant species. [Ca2+](i) oscillations in MIN6 cells recovered when BSA was replaced by buffer or as FA levels in the supernatant were restored. This was achieved by recombinant lipase-mediated FA liberation from membrane lipids, by the addition of FA-preloaded FAF-BSA, or by the photolysis of cell-impermeant caged FAs. Our combined data support the hypothesis of FAs as essential endogenous signaling factors for beta-cell activity and insulin secretion. Pracoviště Ústav organické chemie a biochemie Kontakt asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418 Rok sběru 2019
Počet záznamů: 1