Počet záznamů: 1  

Endogenous Fatty Acids Are Essential Signaling Factors of Pancreatic beta-Cells and Insulin Secretion

  1. 1.
    SYSNO ASEP0495209
    Druh ASEPJ - Článek v odborném periodiku
    Zařazení RIVJ - Článek v odborném periodiku
    Poddruh JČlánek ve WOS
    NázevEndogenous Fatty Acids Are Essential Signaling Factors of Pancreatic beta-Cells and Insulin Secretion
    Tvůrce(i) Hauke, S. (DE)
    Keutler, K. (DE)
    Phapale, P. (DE)
    Yushchenko, Dmytro A. (UOCHB-X) ORCID, RID
    Schultz, C. (DE)
    Zdroj.dok.Diabetes. - : American Diabetes Association - ISSN 0012-1797
    Roč. 67, č. 10 (2018), s. 1986-1998
    Poč.str.13 s.
    Jazyk dok.eng - angličtina
    Země vyd.US - Spojené státy americké
    Klíč. slovahuman plasma albumin ; cytoplasmic Ca2+ ; serum albumin
    Vědní obor RIVCE - Biochemie
    Obor OECDBiochemistry and molecular biology
    Institucionální podporaUOCHB-X - RVO:61388963
    UT WOS000445059100008
    EID SCOPUS85054776125
    DOI10.2337/db17-1215
    AnotaceThe secretion of insulin from beta-cells depends on extracellular factors, in particular glucose and other small molecules, some of which act on G-protein-coupled receptors. Fatty acids (FAs) have been discussed as exogenous secretagogues of insulin for decades, especially after the FA receptor GPR40 (G-protein-coupled receptor 40) was discovered. However, the role of FAs as endogenous signaling factors has not been investigated until now. In the present work, we demonstrate that lowering endogenous FA levels in beta-cell medium by stringent washing or by the application of FA-free (FAF) BSA immediately reduced glucose-induced oscillations of cytosolic Ca2+ ([Ca2+](i) oscillations) in MIN6 cells and mouse primary beta-cells, as well as insulin secretion. Mass spectrometry confirmed BSA-mediated removal of FAs, with palmitic, stearic, oleic, and elaidic acid being the most abundant species. [Ca2+](i) oscillations in MIN6 cells recovered when BSA was replaced by buffer or as FA levels in the supernatant were restored. This was achieved by recombinant lipase-mediated FA liberation from membrane lipids, by the addition of FA-preloaded FAF-BSA, or by the photolysis of cell-impermeant caged FAs. Our combined data support the hypothesis of FAs as essential endogenous signaling factors for beta-cell activity and insulin secretion.
    PracovištěÚstav organické chemie a biochemie
    Kontaktasep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418
    Rok sběru2019
Počet záznamů: 1  

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