Počet záznamů: 1
Light scattering on PHA granules protects bacterial cells against the harmful effects of UV radiation
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SYSNO ASEP 0494657 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Light scattering on PHA granules protects bacterial cells against the harmful effects of UV radiation Tvůrce(i) Slaninova, E. (CZ)
Sedláček, P. (CZ)
Mravec, F. (CZ)
Müllerová, L. (CZ)
Samek, Ota (UPT-D) RID, ORCID, SAI
Koller, M. (AT)
Hesko, O. (CZ)
Kučera, D. (CZ)
Márová, I. (CZ)
Obruča, S. (CZ)Celkový počet autorů 10 Zdroj.dok. Applied Microbiology and Biotechnology. - : Springer - ISSN 0175-7598
Roč. 102, č. 4 (2018), s. 1923-1931Poč.str. 9 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. DE - Německo Klíč. slova polyhydroxyalkanoates ; cupriavidus necator ; UV radiation ; turbidity ; integrating sphere ; nephelometry Vědní obor RIV BH - Optika, masery a lasery Obor OECD Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials CEP GA15-20645S GA ČR - Grantová agentura ČR Institucionální podpora UPT-D - RVO:68081731 UT WOS 000424053700033 EID SCOPUS 85040786575 DOI 10.1007/s00253-018-8760-8 Anotace Numerous prokaryotes accumulate polyhydroxyalkanoates (PHA) in the form of intracellular granules. The primary function of PHA is the storage of carbon and energy. Nevertheless, there are numerous reports that the presence of PHA granules in microbial cells enhances their stress resistance and fitness when exposed to various stress factors. In this work, we studied the protective mechanism of PHA granules against UV irradiation employing Cupriavidus necator as a model bacterial strain. The PHA-accumulating wild type strain showed substantially higher UV radiation resistance than the PHA non-accumulating mutant. Furthermore, the differences in UV-Vis radiation interactions with both cell types were studied using various spectroscopic approaches (turbidimetry, absorption spectroscopy, and nephelometry). Our results clearly demonstrate that intracellular PHA granules efficiently scatter UV radiation, which provides a substantial UV-protective effect for bacterial cells and, moreover, decreases the intracellular level of reactive oxygen species in UV-challenged cells. The protective properties of the PHA granules are enhanced by the fact that granules specifically bind to DNA, which in turn provides shield-like protection of DNA as the most UV-sensitive molecule. To conclude, the UV-protective action of PHA granules adds considerable value to their primary storage function, which can be beneficial in numerous environments. Pracoviště Ústav přístrojové techniky Kontakt Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Rok sběru 2019
Počet záznamů: 1