Počet záznamů: 1
Early and progressive microstructural brain changes in mice overexpressing human alpha-Synuclein detected by diffusion kurtosis imaging
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SYSNO ASEP 0477748 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Early and progressive microstructural brain changes in mice overexpressing human alpha-Synuclein detected by diffusion kurtosis imaging Tvůrce(i) Khairnar, A. (CZ)
Rudá-Kučerová, J. (CZ)
Szabó, N. (HU)
Dražanová, Eva (UPT-D) RID, ORCID, SAI
Arab, A. (CZ)
Hutter-Paier, B. (AT)
Neddens, J. (AT)
Latta, P. (CZ)
Starčuk jr., Zenon (UPT-D) RID, ORCID, SAI
Rektorová, I. (CZ)Celkový počet autorů 10 Zdroj.dok. Brain Behavior and Immunity. - : Elsevier - ISSN 0889-1591
Roč. 61, MAR (2017), s. 197-208Poč.str. 12 s. Forma vydání Tištěná - P Jazyk dok. eng - angličtina Země vyd. CA - Kanada Klíč. slova MRI ; diffusion kurtosis imaging ; substantia nigra ; sriatum ; thalamus ; TNWT-61 ; parkinson's disease ; transgenic mice ; animal model Vědní obor RIV BH - Optika, masery a lasery Obor OECD 1.7 Other natural sciences CEP LO1212 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy LM2015062 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy ED0017/01/01 GA MŠMT - Ministerstvo školství, mládeže a tělovýchovy Institucionální podpora UPT-D - RVO:68081731 UT WOS 000395365900022 EID SCOPUS 85010773884 DOI 10.1016/j.bbi.2016.11.027 Anotace Diffusion kurtosis imaging (DKI) is sensitive in detecting alpha-Synuclein (alpha-Syn) accumulation-associated microstructural changes at late stages of the pathology in alpha-Syn overexpressing TNWT-61 mice. The aim of this study was to perform DKI in young TNWT-61 mice when alpha-Syn starts to accumulate and to compare the imaging results with an analysis of motor and memory impairment and alpha-Syn levels. Three-month-old (3mo) and six-month-old (6mo) mice underwent DIU scanning using the Bruker Avance 9.4 T magnetic resonance imaging system. Region of interest (ROI) analyses were performed in the gray matter, tract-based spatial statistics (TBSS) analyses were performed in the white matter. In the same mice, alpha-Syn expression was evaluated using quantitative immunofluorescence. Mean kurtosis (MK) was the best differentiator between TNWT-61 mice and wildtype (WT) mice. We found increases in MK in 3mo TNWT-61 mice in the striatum and thalamus but not in the substantia nigra (SN), hippocampus, or sensorimotor cortex, even though the immunoreactivity of human alpha-Syn was similar or even higher in the latter regions. Increases in MK in the SN were detected in 6mo mice. These findings indicate that alpha-Syn accumulation-associated changes may start in areas with a high density of dopaminergic nerve terminals. We also found TBSS changes in white matter only at 6mo, suggesting alpha-Syn accumulation-associated changes start in the gray matter and later progress to the white matter. MK is able to detect microstructural changes induced by alpha-Syn overexpression in TNWT-61 mice and could be a useful clinical tool for detecting early-stage Parkinson's disease in human patients. Pracoviště Ústav přístrojové techniky Kontakt Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Rok sběru 2018
Počet záznamů: 1