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Poly(amino acid)-based fibrous scaffolds modified with surface-pendant peptides for cartilage tissue engineering
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SYSNO ASEP 0472812 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Poly(amino acid)-based fibrous scaffolds modified with surface-pendant peptides for cartilage tissue engineering Tvůrce(i) Svobodová, Jana (UMCH-V) RID, ORCID
Proks, Vladimír (UMCH-V) RID, ORCID
Karabiyik, Ö. (TR)
Calikoglu Koyuncu, A. C. (TR)
Köse, G. T. (TR)
Rypáček, František (UMCH-V) RID
Studenovská, Hana (UMCH-V) RID, ORCIDZdroj.dok. Journal of Tissue Engineering and Regenerative Medicine. - : Wiley - ISSN 1932-6254
Roč. 11, č. 3 (2017), s. 831-842Poč.str. 12 s. Jazyk dok. eng - angličtina Země vyd. GB - Velká Británie Klíč. slova poly(amino acid) ; fibrous scaffolds ; adhesion peptide Vědní obor RIV CD - Makromolekulární chemie Obor OECD Polymer science CEP GAP108/12/1629 GA ČR - Grantová agentura ČR GAP108/12/1538 GA ČR - Grantová agentura ČR Institucionální podpora UMCH-V - RVO:61389013 UT WOS 000398046600023 EID SCOPUS 84921534324 DOI 10.1002/term.1982 Anotace In this study, fibrous scaffolds based on poly(gamma-benzyl-l-glutamate) (PBLG) were investigated in terms of the chondrogenic differentiation potential of human tooth germ stem cells (HTGSCs). Through the solution-assisted bonding of the fibres, fully connected scaffolds with pore sizes in the range 20–400 µm were prepared. Biomimetic modification of the PBLG scaffolds was achieved by a two-step reaction procedure: first, aminolysis of the PBLG fibres’ surface layers was performed, which resulted in an increase in the hydrophilicity of the fibrous scaffolds after the introduction of N5-hydroxyethyl-l-glutamine units. Second, modification with the short peptide sequence azidopentanoyl–GGGRGDSGGGY–NH2, using the 'click' reaction on the previously modified scaffold with 2-propynyl side-chains, was performed. Radio-assay of the 125I-labelled peptide was used to evaluate the RGD density in the fibrous scaffolds (which varied in the range 10–3–10 pm/cm2). All the PBLG scaffolds, especially with density 90 ± 20 fm/cm2 and 200 ± 100 fm/cm2 RGD, were found to be potentially suitable for growth and chondrogenic differentiation of HTGSCs. Pracoviště Ústav makromolekulární chemie Kontakt Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Rok sběru 2018
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