Počet záznamů: 1
Proteomic analysis of protein composition of rat forebrain cortex exposed to morphine for 10 days; comparison with animals exposed to morphine and subsequently nurtured for 20 days in the absence of this drug
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SYSNO ASEP 0461865 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Proteomic analysis of protein composition of rat forebrain cortex exposed to morphine for 10 days; comparison with animals exposed to morphine and subsequently nurtured for 20 days in the absence of this drug Tvůrce(i) Ujčíková, Hana (FGU-C) RID, ORCID
Vošahlíková, Miroslava (FGU-C) RID, ORCID, SAI
Roubalová, Lenka (FGU-C) RID, ORCID, SAI
Svoboda, Petr (FGU-C) RID, ORCIDZdroj.dok. Journal of Proteomics. - : Elsevier - ISSN 1874-3919
Roč. 145, Aug 11 (2016), s. 11-23Poč.str. 13 s. Jazyk dok. eng - angličtina Země vyd. NL - Nizozemsko Klíč. slova morphine ; long-term exposure ; rat forebrain cortex ; post-nuclear supernatant ; MALDI-TOF MS/MS ; MaxLFQ Vědní obor RIV CE - Biochemie CEP GBP304/12/G069 GA ČR - Grantová agentura ČR GAP207/12/0919 GA ČR - Grantová agentura ČR Institucionální podpora FGU-C - RVO:67985823 UT WOS 000383931000003 EID SCOPUS 84959499628 DOI 10.1016/j.jprot.2016.02.019 Anotace Proteomic analysis was performed in post-nuclear supernatant fraction (PNS) prepared from forebrain cortex of rats exposed to increasing doses of morphine (10–50 mg/kg) for 10 days and sacrificed 24 h (group + M10) or 20 days (group + M10/−M20) after the last dose of morphine. PNS fraction was resolved by 2D-ELFO and stained by CBB. Analysis of the difference between (+ M10) and (− M10) samples of PNS by PDQuest accompanied by MALDI-TOF MS/MS indicated the significant change of 28 proteins. Importantly, the number of altered proteins was decreased to 14 after 20 days of nurturing animals in the absence of morphine. This new and important finding indicating the ability of mammalian organism to return to physiological norm after removal of the drug was verified by an independent methodology — gel-free & label-free quantification and normalization procedure denominated as MaxLFQ. The 113 proteins were identified as altered by morphine in (+ M10) samples when compared with (− M10) samples of PNS and this number was decreased to 19 after 20 days of nurturing the animals in the absence of this drug. Pracoviště Fyziologický ústav Kontakt Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Rok sběru 2017
Počet záznamů: 1