Počet záznamů: 1
Transcriptional response of lignin-degrading enzymes to 17 alpha-ethinyloestradiol in two white rots
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SYSNO ASEP 0399070 Druh ASEP J - Článek v odborném periodiku Zařazení RIV J - Článek v odborném periodiku Poddruh J Článek ve WOS Název Transcriptional response of lignin-degrading enzymes to 17 alpha-ethinyloestradiol in two white rots Tvůrce(i) Přenosilová, Lenka jr. (MBU-M)
Křesinová, Zdena (MBU-M) RID
Slavíková-Anemori, Anna (MBU-M)
Cajthaml, Tomáš (MBU-M) RID, ORCID
Svobodová, Kateřina (MBU-M) RIDZdroj.dok. Microbial Biotechnology - ISSN 1751-7907
Roč. 6, č. 3 (2013), s. 300-306Poč.str. 6 s. Jazyk dok. eng - angličtina Země vyd. US - Spojené státy americké Klíč. slova LACCASE GENE-EXPRESSION ; TRAMETES SP AH28-2 ; MANGANESE PEROXIDASE Vědní obor RIV EE - Mikrobiologie, virologie CEP GAP503/10/0408 GA ČR - Grantová agentura ČR TA01020804 GA TA ČR - Technologická agentura ČR Institucionální podpora MBU-M - RVO:61388971 UT WOS 000320186500010 DOI 10.1111/1751-7915.12007 Anotace Fungal, ligninolytic enzymes have attracted a great attention for their bioremediation capabilities. A deficient knowledge of regulation of enzyme production, however, hinders the use of ligninolytic fungi in bioremediation applications. In this work, a transcriptional analyses of laccase and manganese peroxidase (MnP) production by two white rots was combined with determination of pI of the enzymes and the evaluation of 17 alpha-ethinyloestradiol (EE2) degradation to study regulation mechanisms used by fungi during EE2 degradation. In the cultures of Trametes versicolor the addition of EE2 caused an increase in laccase activity with a maximum of 34.2 +/- 6.7 U g(-1) of dry mycelia that was observed after 2 days of cultivation. It corresponded to a 4.9 times higher transcription levels of a laccase-encoding gene (lacB) that were detected in the cultures at the same time. Simultaneously, pI values of the fungal laccases were altered in response to the EE2 treatment. Like T. versicolor, Irpex lacteus was also able to remove 10 mg l(-1) EE2 within 3 days of cultivation. While an increase to I. lacteus MnP activity and MnP gene transcription levels was observed at the later phase of the cultivation. It suggests another metabolic role of MnP but EE2 degradation Pracoviště Mikrobiologický ústav Kontakt Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Rok sběru 2014
Počet záznamů: 1